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dc.contributor.authorMuñoz Odina, Mª Pilar
dc.contributor.authorLópez Alonso, Carmen
dc.contributor.authorMoralejo Vidal, Mª Angeles
dc.contributor.authorPérez Hedo, Meritxell
dc.contributor.authorEizaguirre Altuna, Matilde
dc.date.accessioned2015-11-13T11:14:28Z
dc.date.available2015-11-13T11:14:28Z
dc.date.issued2014
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10459.1/48957
dc.description.abstractBt crops are able to produce Cry proteins, which were originally present in Bacillus thuringiensis bacteria. Although Bt maize is very efficient against corn borers, Spanish crops are also attacked by the earworm H. armigera, which is less susceptible to Bt maize. Many mechanisms could be involved in this low susceptibility to the toxin, including the insect’s metabolic resistance to toxins due to cytochrome P450 monooxygenases. This paper examines the response of last instar H. armigera larvae to feeding on a diet with Bt and non-Bt maize leaves in larval development and in the gene expression of three P450 cytochromes: CYP6AE14, CYP6B2 and CYP9A12. Larvae fed on sublethal amounts of the Bt toxin showed reduced food ingestion and reduced growth and weight, preventing most of them from achieving the critical weight and pupating; additionally, after feeding for one day on the Bt diet the larvae showed a slight increase in juvenile hormone II in the hemolymp. Larvae fed on the non-Bt diet showed the highest CYP6AE14, CYP6B2 and CYP9A12 expression one day after feeding on the non-Bt diet, and just two days later the expression decreased abruptly, a finding probably related to the developmental programme of the last instar. Moreover, although the response of P450 genes to plant allelochemicals and xenobiotics has been related in general to overexpression in the resistant insect, or induction of the genes when feeding takes place, the expression of the three genes studied was suppressed in the larvae feeding on the Bt toxin. The unexpected inhibitory effect of the Cry1Ab toxin in the P450 genes of H. armigera larvae should be thoroughly studied to determine whether this response is somehow related to the low susceptibility of the species to the Bt toxin.ca_ES
dc.description.sponsorshipThis work was supported by a grant from the Spanish R&D Agency (Comision Interministerial de Ciencia y Tecnologıa) through project AGL2011-23996
dc.language.isoengca_ES
dc.publisherPublic Library of Scienceca_ES
dc.relationMICINN/PN2008-2011/AGL2011-23996
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1371/journal.pone.0099229ca_ES
dc.relation.ispartofPLoS ONE, 2014, vol. 9, núm. 6, e99229ca_ES
dc.rightscc-by (c) Muñoz Odina, Mª Pilar et al., 2014ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.titleResponse of Last Instar Helicoverpa armígera Larvae to Bt Toxin Ingestion: Changes in the Development and in the CYP6AE14, CYP6B2 and CYP9A12 Gene Expressionca_ES
dc.typearticleca_ES
dc.identifier.idgrec021285
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0099229


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cc-by (c) Muñoz Odina, Mª Pilar et al., 2014
Except where otherwise noted, this item's license is described as cc-by (c) Muñoz Odina, Mª Pilar et al., 2014