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dc.contributor.authorPujol Carrión, Núria
dc.contributor.authorSato, Mamiko
dc.contributor.authorPinar, Mario
dc.contributor.authorRamos, Mariona
dc.contributor.authorMoreno, Belén
dc.contributor.authorOsumi, Masako
dc.contributor.authorRibas, Juan Carlos
dc.contributor.authorPérez, Pilar
dc.contributor.authorCortés, Juan C. G.
dc.date.accessioned2015-11-11T12:05:13Z
dc.date.available2015-11-11T12:05:13Z
dc.date.issued2015
dc.identifier.issn1553-7390
dc.identifier.urihttp://hdl.handle.net/10459.1/48951
dc.description.abstractIn fungal cells cytokinesis requires coordinated closure of a contractile actomyosin ring (CAR) and synthesis of a special cell wall structure known as the division septum. Many CAR proteins have been identified and characterized, but how these molecules interact with the septum synthesis enzymes to form the septum remains unclear. Our genetic study using fission yeast shows that cooperation between the paxillin homolog Pxl1, required for ring integrity, and Bgs1, the enzyme responsible for linear β(1,3)glucan synthesis and primary septum formation, is required for stable anchorage of the CAR to the plasma membrane before septation onset, and for cleavage furrow formation. Thus, lack of Pxl1 in combination with Bgs1 depletion, causes failure of ring contraction and lateral cell wall overgrowth towards the cell lumen without septum formation. We also describe here that Pxl1 concentration at the CAR increases during cytokinesis and that this increase depends on the SH3 domain of the F-BAR protein Cdc15. In consequence, Bgs1 depletion in cells carrying a cdc15ΔSH3 allele causes ring disassembly and septation blockage, as it does in cells lacking Pxl1. On the other hand, the absence of Pxl1 is lethal when Cdc15 function isaffected, generating a large sliding of the CAR with deposition of septum wall material along the cell cortex, and suggesting additional functions for both Pxl1 and Cdc15 proteins. In conclusion, our findings indicate that CAR anchorage to the plasma membrane through Cdc15 and Pxl1, and concomitant Bgs1 activity, are necessary for CAR maintenance and septum formation in fission yeast.ca_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation (BFU2010-15641 and BFU2013-39394-P) to PP. JCR was financed by the Spanish Ministry of Science and Innovation (BIO2012-35372), and Junta de Castilla y León, Spain (CSI037U14). JCGC was supported by a Juan de la Cierva postdoctoral contract from the Spanish Ministry of Science and Innovation.
dc.language.isoengca_ES
dc.publisherPublic Library of Scienceca_ES
dc.relationMICINN/PN2008-2011/BFU2010-15641
dc.relationMINECO/PN2013-2016/BFU2013-39394-P
dc.relationMICINN/PN2008-2011/BIO2012-35372
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1371/journal.pgen.1005358ca_ES
dc.relation.ispartofPLoS Genetics, 2015, vol. 11, núm. 7ca_ES
dc.rightscc-by (c) G. Cortés, Juan C. et al., 2015ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.titleCooperation between Paxillin-like Protein Pxl1 and Glucan Synthase Bgs1 Is Essential for Actomyosin Ring Stability and Septum Formation in Fission Yeastca_ES
dc.typearticleca_ES
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1371/journal.pgen.1005358


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cc-by (c) G. Cortés, Juan C. et al., 2015
Except where otherwise noted, this item's license is described as cc-by (c) G. Cortés, Juan C. et al., 2015