Metabolite profiling of olive oil and thyme phenols after a sustained intake of two phenol-enriched olive oils by humans: Identification of compliance markers
De la Torre, Rafael
Valls, Rosa M.
MetadataShow full item record
An understanding of causal relations between phenol intake and its beneficial effects on health is hindered by the lack of robust biological markers of its exposure. This is particularly relevant in mid/long-term nutritional intervention studies. An analytical methodology based on UPLC<br>MS/MS has
been developed to determine the metabolites of the phenolic compounds from olive oil and thyme in biological fluids after a sustained intake of two phenol-enriched olive oils for their further use as compliance biomarkers. In a randomized, double-blind, controlled, cross-over trial, 33 hypercholesterolemic volunteers received during 3 weeks 25 mL/day of (1) raw Virgin Olive Oil with a low phenolic content as a control (80 mg total phenols / kg oil; VOO), (2) Functional Virgin Olive Oil enriched with its own phenolics (500 mg total phenols / kg oil; FVOO), and (3) Functional Virgin Olive Oil enriched with its own phenolics plus complementary phenolics from Thyme (500 mg total phenols / kg oil, 50% from olive oil and 50% from thyme respectively; FVOOT). Plasma and 24 h-urine samples were collected. The results showed that somehydroxytyrosol (HT)metabolites presented lowspecificity as biomarkers of intake. However, hydroxytyrosol sulfate and hydroxytyrosol acetate sulfate appeared to be suitable biomarkers for monitoring compliancewith olive oil intake as their values in plasmaor/and 24-h urinewere significantly higher after FVOO compared to baseline pre-intervention concentrations. They were also significantly correlated with the monitored level of compliance. On the other hand, metabolites derived from thyme were more specific, thymol sulfate and hydroxyphenylpropionic acid sulfate being the metabolites with the largest increase in both plasma and 24-h urine, whereas urinary p-cymene-diol glucuronide presented the greatest increase post-treatment. Their urinary excretion values also displayed significant correlations with the level of compliance and they were defined as FVOOT compliance biomarkers. This study enabled robust quantitative and qualitative compliance biomarkers after the ingestion of two phenol-enriched olive oils to be determined and provided a thorough analysis of the true phenolic exposure after a sustained consumption that could be further related to expected biological effects.
Is part ofFood Research International, 2014, vol. 65, p. 59-68
Showing items related by title, author, creator and subject.
Validation of dried blood spot cards to determine apple phenolic metabolites in human blood and plasma after an acute intake of red-fleshed apple snack Yuste, Silvia; Macià i Puig, Ma Alba; Ludwig, Iziar A.; Romero Fabregat, Mª Paz; Fernández Castillejo, Sara; Catalán Santos, Úrsula; Motilva Casado, Mª José; Rubió Piqué, Laura (Wiley, 2018)Scope: The application of dried blood spot (DBS) cards for the study in human blood of dietary polyphenol bioavailability has been poorly studied. Methods and results: An analytical method based on blood sampling with DBS ...
Suárez, Manuel; Valls, Rosa M.; Romero Fabregat, Mª Paz; Macià i Puig, Ma Alba; Fernandez, Sara; Giralt, Montserrat; Solà, Rosa; Motilva Casado, Mª José (Cambridge University Press, 2011)Phenolic compounds are one of the main reasons behind the healthy properties of virgin olive oil (VOO). However, their daily intake from VOO is low compared with that obtained from other phenolic sources. Therefore, the ...
Virgin olive oil enriched with its own phenolic compounds or complemented with thyme improves endothelial function: The potential role of plasmatic fat-soluble vitamins. A double blind, randomized, controlled, cross-over clinical trial Valls, Rosa M.; Farràs, Marta; Pedret, Anna; Fernández Castillejo, Sara; Catalán Santos, Úrsula; Romeu, Marta; Giralt, Montserrat; Sáez, Guillermo T.; Fitó, Montserrat; De la Torre, Rafael; Covas Planells, María Isabel; Motilva Casado, Mª José; Solà, Rosa; Rubió Piqué, Laura (Elsevier, 2017)The aim of the present study was to assess whether different functional virgin olive oils (FVOOs) with varying phenolic compounds (PC) could protect the plasmatic fat-soluble vitamins, which in turn could improve the ...