The application of pulsed light (PL) in combination with malic acid (MA) dips was evaluated regarding the inactivation of Listeria innocua or Escherichia coil inoculated on different fresh-cut products (avocado, watermelon and mushrooms) through refrigerated storage. The inoculated substrates were exposed to individual PL (180-1100 nm, 12 J/cm(2)) and MA (2% w/v) treatments or to a combination of both. The effects on the bacterial cell structure were confirmed by Transmission Electron Microscopy (TEM). When treatments were individually applied, inactivation of L. innocua and E. coli ranged from 0.91 to 1.1 log CFU/g and from 1.92 to 2.97 log CFU/g, respectively. Nevertheless, a much greater bactericidal effect was observed when the treatments were sequentially applied, leading to more than 5 log reductions of L. innocua and E. coli populations. TEM analysis revealed structural changes with modification of cytoplasmic contents and alteration of cell membranes. The results of this study indicate that the combination of assayed treatments may be applied to effectively control L innocua and E. coli on the surface of fresh-cut products for at least two weeks of storage. (C) 2014 Elsevier Ltd. All rights reserved