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dc.contributor.authorMuñoz González, Sara
dc.contributor.authorRuggli, Nicolas
dc.contributor.authorRosell, Rosa
dc.contributor.authorJosue Perez, Lester
dc.contributor.authorFrias Leuporeau, Maria Teresa
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authorMontoya, Maria
dc.contributor.authorCórdoba, Lorena
dc.contributor.authorDomingo, Mariano
dc.contributor.authorEhrensperger, Felix
dc.contributor.authorSummerfield, Artur
dc.contributor.authorGanges, Llilianne
dc.description.abstractIt is well established that trans-placental transmission of classical swine fever virus (CSFV) during mid-gestation can lead to persistently infected offspring. The aim of the present study was to evaluate the ability of CSFV to induce viral persistence upon early postnatal infection. Two litters of 10 piglets each were infected intranasally on the day of birth with low and moderate virulence CSFV isolates, respectively. During six weeks after postnatal infection, most of the piglets remained clinically healthy, despite persistent high virus titres in the serum. Importantly, these animals were unable to mount any detectable humoral and cellular immune response. At necropsy, the most prominent gross pathological lesion was a severe thymus atrophy. Four weeks after infection, PBMCs from the persistently infected seronegative piglets were unresponsive to both, specific CSFV and non-specific PHA stimulation in terms of IFN-gamma-producing cells. These results suggested the development of a state of immunosuppression in these postnatally persistently infected pigs. However, IL-10 was undetectable in the sera of the persistently infected animals. Interestingly, CSFV-stimulated PBMCs from the persistently infected piglets produced IL-10. Nevertheless, despite the addition of the anti-IL-10 antibody in the PBMC culture from persistently infected piglets, the response of the IFN-gamma producing cells was not restored. Therefore, other factors than IL-10 may be involved in the general suppression of the T-cell responses upon CSFV and mitogen activation. Interestingly, bone marrow immature granulocytes were increased and targeted by the virus in persistently infected piglets. Taken together, we provided the first data demonstrating the feasibility of CSFV in generating a postnatal persistent disease, which has not been shown for other members of the Pestivirus genus yet. Since serological methods are routinely used in CSFV surveillance, persistently infected pigs might go unnoticed. In addition to the epidemiological and economic significance of persistent CSFV infection, this model could be useful for understanding the mechanisms of viral persistence.ca_ES
dc.description.sponsorshipThis research was supported by grant AGL2012-38343 from Spanish government. Author who received the funding: Llilianne Ganges (LG). S. Muñoz-González received predoctoral fellowship FI-DGR 2014 from AGAUR, Generalitat de Catalunya.
dc.publisherPublic Library Scienceca_ES
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofPLoS One, 2015, vol. 10, núm.5, e0125692ca_ES
dc.rightscc-by, (c) Muñoz González, Sara et al., 2015ca_ES
dc.subjectConventional dentritic cellsca_ES
dc.subjectRespiratory syndrome virusca_ES
dc.subjectHog-cholera virusca_ES
dc.subjectDescriptive epidemiologyca_ES
dc.subject.otherCèl·lules dendrítiquesca_ES
dc.titlePostnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implicationsca_ES

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cc-by, (c) Muñoz González, Sara et al., 2015
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