Prokaryotic and eukaryotic monothiol glutaredoxins are able to perform the functions of Grx5 in the biogenesis of Fe/S clusters in yeast mitochondria
Molina Navarro, Maria Micaela
MetadataShow full item record
The Saccharomyces cerevisiae monothiol glutare- doxin Grx5 participates in the mitochondrial biogenesis of iron–sulfur clusters. Grx5 homologues exist in organisms from bacteria to humans. Chicken (cGRX5) and human (hGRX5) homologues contain a mitochondrial targeting sequence, suggest- ing a mitochondrial localization for these two proteins. We have compartmentalized the Escherichia coli and Synechocystis sp. homologues, and also cGRX5 and hGRX5, in the mitochondrial matrix of a yeast grx5 mutant. All four heterologous proteins rescue the defects of the mutant. The chicken cGRX5 gene was significantly expressed throughout the embryo stages in different tissues. These results underline the functional conservation of Grx5 homologues throughout evolution.
Is part ofFEBS Letters, 2006, vol. 580, p. 2273-2280
European research projects
Showing items related by title, author, creator and subject.
Nuclear monothiol glutaredoxins of Saccharomyces cerevisiae can function as mitochondrial glutaredoxins Molina Navarro, Maria Micaela; Bellí i Martínez, Gemma; Torre Ruiz, M. A. de la; Rodríguez Manzanaque, Maria Teresa; Herrero Perpiñán, Enrique (The American Society for Biochemistry and Molecular Biology, 2004)Glutaredoxins are thiol oxidoreductases that regulate protein redox state. In Saccharomyces cerevisiae, Grx1 and Grx2 are cytosolic dithiol glutaredoxins, whereas Grx3, Grx4, and Grx5 are monothiol glutaredoxins. ...
Cytosolic monothiol glutaredoxins function in intracellular iron sensing and trafficking via their bound iron-sulfur cluster Mühlenhoff, Ulrich; Molik, Sabine; Godoy, José R.; Uzarska, Marta A.; Richter, Nadine; Seubert, Andreas; Zhang, Yan; Stubbe, JoAnne; Pierrel, Fabien; Herrero Perpiñán, Enrique; Lillig, Christopher Horst; Lill, Roland (Elsevier, 2010)Iron is an essential nutrient for cells. It is unknown how iron, after its import into the cytosol, is speciﬁcally delivered to iron-dependent processes in various cellular compartments. Here, we identify an essential ...
Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma; Casas Herranz, Celia (Bentham Science Publishers, 2010)Glutaredoxins are defined as thiol disulfide oxidoreductases that reduce disulfide bonds employing reduced glutathione as electron donor. They constitute a complex family of proteins with a diversity of enzymatic and ...