Characterization of a Candida albicans gene encoding a putative transcriptional factor required for cell wall integrity
Maicas i Prieto, Sergio
MetadataShow full item record
After screening a Candida albicans genome database the product of an open reading frame (ORF) (CA2880) with 49% homology to the product of Saccharomyces cerevisiae YPL133c, a putative transcriptional factor, was identified. The disruption of the C. albicans gene leads to a major sensitivity to calcofluor
white and Congo red, a minor sensitivity to sodium dodecyl sulfate, a major resistance to zymolyase, and an alteration of the chemical composition of the cell wall. For these reasons we called it CaCWT1 (for C. albicans cell wall transcription factor). CaCwt1p contains a putative Zn(II) Cys(6) DNA binding domain characteristic of some transcriptional factors and a PAS domain. The CaCWT1 gene is more expressed in stationary phase cells than in cells growing exponentially. To our knowledge, this is the first Zn(II) Cys(6) transcriptional factor-encoding gene implicated in the cell wall architecture.
Is part ofFEMS Microbiology Letters, 2003, vol. 226, p. 159-167
European research projects
Showing items related by title, author, creator and subject.
Garcera, Ana; Castillo, Luis; Martínez, Ana Isabel; Elorza, M. Victoria; Valentín, Eulogio; Sentandreu, Rafael (Elsevier, 2005-06-22)Incorporation into the wall of Candida albicans Ssr1, a GPI-dependent protein, was investigated by construction of different truncated genes for which the three potential ω sites (S199, S215 and G216) and the corresponding ...
Isolation of a Candida albicans gene, tightly linked to URA3, coding for a putative transcription factor that suppresses a Saccharomyces cerevisiae aft1 mutation Gómez García, Micaela; O'Connor, José-Enrique; Latorre García, Lorena; Irar Martínez, Sami; Herrero Perpiñán, Enrique; Del Castillo Agudo, Lucas (John Wiley & Sons, 2001)A pathogen such as C. albicans needs an ef®cient mechanism of iron uptake in an iron- restricted environment such as is the human body. A ferric-reductase activity regulated by iron and copper, and analogous to that in ...
Castillo, Luis; Martínez, Ana Isabel; Garcera, Ana; García-Martínez, José; Ruiz-Herrera, José; Valentín, Eulogio; Sentandreu, Rafael (Elsevier, 2005-12-16)Transcription proWling of Candida albicans cells responding to the elimination of the wall (protoplasts) and posterior regeneration was explored. DNA microarrays were used to measure changes in the expression of 6039 genes, ...