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dc.contributor.authorBellí i Martínez, Gemma
dc.contributor.authorGarí Marsol, Eloi
dc.contributor.authorAldea, Martí
dc.contributor.authorHerrero Perpiñán, Enrique
dc.date.accessioned2015-07-16T07:51:02Z
dc.date.issued2001
dc.identifier.issn1365-2958
dc.identifier.urihttp://hdl.handle.net/10459.1/48602
dc.description.abstractModerate hyperosmotic stress on Saccharomyces cerevisiae cells produces a temporary delay at the G1 stage of the cell cycle. This is accompanied by transitory downregulation of CLN1, CLN2 and CLB5 transcript levels, although not of CLN3, which codes for the most upstream activator of the G1/S transition. Osmotic shock to cells synchronized in early G1, when Cln3 is the only cyclin present, causes a delay in cell cycle resumption. This points to Cln3 as being a key cell cycle target for osmotic stress. We have observed that osmotic shock causes downregulation of the kinase activity of Cln3±Cdc28 complexes. This is concomitant with a temporary accumulation of Cln3 protein as a result of increased stability. The effects of the osmotic stress in G1 are not suppressed in CLN3-1 cells with increased kinase activity, as the Cln3±Cdc28 activity in this mutant is still affected by the shock. Although Hog1 is not required for the observed cell cycle arrest in hyperosmotic conditions, it is necessary to resume the cell cycle at KCl concentrations higher than 0.4 M.ca_ES
dc.language.isoengca_ES
dc.publisherBlackwell Scienceca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1046/j.1365-2958.2001.02297.xca_ES
dc.relation.ispartofMolecular Microbiology, 2001, vol. 39, núm. 4, p. 1022-1035ca_ES
dc.rights(c) Blackwell Science, 2001ca_ES
dc.titleOsmotic stress causes a G1 cell cycle delay and downregulation of Cln3/Cdc28 activity in Saccharomyces cerevisiaeca_ES
dc.typearticleca_ES
dc.identifier.idgrec004081
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1046/j.1365-2958.2001.02297.x
dc.date.embargoEndDate2025-01-01


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