Wound response in orange as a resistance mechanism against Penicillium digitatum (pathogen) and P. expansum (non-host pathogen)

Thumbnail
View/Open
Issue date
2013
Author
Vilanova, Laura
Torres Sanchis, Rosario
Viñas Almenar, Inmaculada
González-Candelas, Luis
Usall i Rodié, Josep
Fiori, S.
Solsona Aixalà, Cristina
Teixidó i Espasa, Neus
Suggested citation
Vilanova, Laura; Torres Sanchis, Rosario; Viñas Almenar, Inmaculada; González-Candelas, Luis; Usall i Rodié, Josep; Fiori, S.; ... Teixidó i Espasa, Neus. (2013) . Wound response in orange as a resistance mechanism against Penicillium digitatum (pathogen) and P. expansum (non-host pathogen). Postharvest Biology and Technology, 2013, vol. 78, p. 113-122. https://doi.org/10.1016/j.postharvbio.2012.12.013.
Impact

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
Penicillium digitatum is the most devastating postharvest pathogen of citrus. In addition, Penicillium expansum is the main pathogen of pome fruit, although recent studies have demonstrated its ability to infect oranges under some conditions. In this study, we evaluated wound response in"Valencia" oranges
harvested at three different maturity stages and the effect of wound response on the establishment of both pathogens when fruit were stored at two different temperatures (20 and 4 °C). The effect of wounding and pathogen inoculation on lignin content, was also quantified. Lastly, the expression of several phenylpropanoid pathway-related genes was also analyzed by semi-quantitative RT-PCR. Results indicated that, in general, P. digitatum exhibited lower decay incidence and severity as time between wounding and inoculation increased. Decay incidence and severity were higher in fruit from the over-mature harvest than in fruit from immature and commercial harvests. P. expansum was able to infect fruit at 20 °C but lesions were small compared to lesion size of fruit stored at 4 °C. Lignin content in wounded fruit (control) and in samples wounded and inoculated with P. expansum was highest in fruit from the immature harvest at 7 d post-wounding and inoculation. Wounded fruit had higher expression of pal1, comt1 and pox1 genes at 48 h than at 24 h. However, samples inoculated with P. digitatum showed lower expression at 48 h than at 24 h. Our results indicated that maturity and storage temperature play an important role in orange wound response.
URI
http://hdl.handle.net/10459.1/48595
DOI
https://doi.org/10.1016/j.postharvbio.2012.12.013
Is part of
Postharvest Biology and Technology, 2013, vol. 78, p. 113-122
Collections