Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae

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2002Author
Ariño Carmona, Joaquín
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Torre Ruiz, M. A. de la;
Torres Rosell, Jordi;
Ariño Carmona, Joaquín;
Herrero Perpiñán, Enrique;
.
(2002)
.
Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae.
The Journal of Biological Chemistry, 2002, vol. 277, núm 36, p. 33468-33476.
https://doi.org/10.1074/jbc.M203515200.
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Show full item recordAbstract
Maintenance of cellular integrity in Saccharomyces
cerevisiae is carried out by the activation of the protein
kinase C-mediated mitogen-activated protein kinase
(PKC1-MAPK) pathway. Here we report that correct
down-regulation of both basal and induced activity of
the PKC1-MAPK pathway requires the SIT4 function.
Sit4 is a protein phosphatase also required for a proper
cell cycle progression. We present evidence demonstrat-
ing that the G1 to S delay in the cell cycle, which occurs
as a consequence of the absence of Sit4, is mediated by
up-regulation of Pkc1 activity. Sit4 operates down-
stream of the plasma membrane sensors Mid2, Wsc1, and
Wsc2 and upstream of Pkc1. Sit4 affects all known bio-
logical functions involving Pkc1, namely Mpk1 activity
and cell wall integrity, actin cytoskeleton organization,
and ribosomal gene transcription.