Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stress

Thumbnail
View/Open
004629.pdf (909.4Kb)
Issue date
2005
Author
Vilella Mitjana, Felipe
Herrero Perpiñán, Enrique
Torres Rosell, Jordi
Torre Ruiz, M. A. de la
Suggested citation
Vilella Mitjana, Felipe; Herrero Perpiñán, Enrique; Torres Rosell, Jordi; Torre Ruiz, M. A. de la; . (2005) . Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stress. The Journal of Biological Chemistry, 2005, vol. 280, núm 10, p. 9149-9159. https://doi.org/10.1074/jbc.M411062200.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
In this study we analyze the participation of the PKC1-MAPK cell integrity pathway in cellular re- sponses to oxidative stress in Saccharomyces cerevisiae. Evidence is presented demonstrating that only Pkc1 and the upstream elements of the cell integrity pathway are essential for cell survival upon treatment with two oxidizing agents, diamide and hydrogen peroxide. Mtl1 is characterized for the first time as a cell-wall sensor of oxidative stress. We also show that the actin cytoskele- ton is a cellular target for oxidative stress. Both diamide and hydrogen peroxide provoke a marked depolariza- tion of the actin cytoskeleton, being Mtl1, Rom2 and Pkc1 functions all required to restore the correct actin organization. Diamide induces the formation of disul- fide bonds in newly secreted cell-wall proteins. This mainly provokes structural changes in the cell outer layer, which activate the PKC1-MAPK pathway and hence the protein kinase Slt2. Our results led us to the conclusion that Pkc1 activity is required to overcome the effects of oxidative stress by: (i) enhancing the ma- chinery required to repair the altered cell wall and (ii) restoring actin cytoskeleton polarity by promoting actin cable formation.
URI
http://hdl.handle.net/10459.1/48444
DOI
https://doi.org/10.1074/jbc.M411062200
Is part of
The Journal of Biological Chemistry, 2005, vol. 280, núm 10, p. 9149-9159
European research projects
Collections
  • Articles publicats (Ciències Mèdiques Bàsiques) [550]
  • Articles publicats (IRBLleida) [1060]

Contact Us | Send Feedback | Legal Notice
© 2022 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2022 BiD. Universitat de Lleida
Metadata subjected to