Transcriptomic responses of Phanerochaete chrysosporium to oak acetonic extracts: focus on a new glutathione transferase.

Thuillier, Anne; Chibani, Kamel; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique; Dumarçay, Stéphane; Gérardin, Philippe; Kohler, Annegret; Deroy, Aurélie; Dhalleine, Tiphaine; Bchini, Raphael; Jacquot, Jean Pierre; Gelhaye, Eric; Morel-Rouhier, Mélanie

doi:https://doi.org/10.1128/AEM.02103-14

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Issue date

2014

Author

Thuillier, Anne

Chibani, Kamel

Bellí i Martínez, Gemma

Herrero Perpiñán, Enrique

Dumarçay, Stéphane

Gérardin, Philippe

Kohler, Annegret

Deroy, Aurélie

Dhalleine, Tiphaine

Bchini, Raphael

Jacquot, Jean Pierre

Gelhaye, Eric

Morel-Rouhier, Mélanie

Suggested citation

Thuillier, Anne; Chibani, Kamel; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique; Dumarçay, Stéphane; Gérardin, Philippe; ... Morel-Rouhier, Mélanie. (2014) . Transcriptomic responses of Phanerochaete chrysosporium to oak acetonic extracts: focus on a new glutathione transferase.. Applied and Environmental Microbiology, 2014, vol. 80, núm. 20, p. 6316-6327. https://doi.org/10.1128/AEM.02103-14.

Abstract

The first steps of wood degradation by fungi lead to the release of toxic compounds known as extractives. To better understand how lignolytic fungi cope with the toxicity of these molecules, a transcriptomic analysis of Phanerochaete chrysosporium genes was performed in presence of oak acetonic extracts. It reveals that in complement to the extracellular machinery of degradation, intracellular antioxidant and detoxification systems contribute to the lignolytic capabilities of fungi presumably by preventing cellular damages and maintaining fungal health. Focusing on these systems, a glutathione transferase (PcGTT2.1) has been selected for functional characterization. This enzyme, not characterized so far in basidiomycetes, has been first classified as a GTT2 in comparison to the Saccharomyces cerevisiae isoform. However, a deeper analysis shows that GTT2.1 isoform has functionally evolved to reduce lipid peroxidation by recognizing high-molecular weight peroxides as substrates. Moreover, the GTT2.1 gene has been lost in some non-wood decay fungi. This example suggests that the intracellular detoxification system could have evolved concomitantly with the extracellular ligninolytic machinery in relation to the capacity of fungi to degrade wood.

URI

http://hdl.handle.net/10459.1/48422

DOI

https://doi.org/10.1128/AEM.02103-14

Is part of

Applied and Environmental Microbiology, 2014, vol. 80, núm. 20, p. 6316-6327