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dc.contributor.authorCastells i Roca, Laia
dc.contributor.authorMühlenhoff, Ulrich
dc.contributor.authorLill, Roland
dc.contributor.authorHerrero Perpiñán, Enrique
dc.contributor.authorBellí i Martínez, Gemma
dc.date.accessioned2015-07-07T10:58:12Z
dc.date.issued2011
dc.identifier.issn0950-382X
dc.identifier.urihttp://hdl.handle.net/10459.1/48421
dc.description.abstractSaccharomyces cerevisiae can import iron through a high-affinity system consisting of the Ftr1/Fet3- mediated reductive pathway and the siderophoremediated non-reductive one. Expression of components of the high-affinity system is controlled by the Aft1 transcriptional factor. In this study we show that, upon oxidative stress, Aft1 is transitorily internalized into the nucleus, followed by transcription activation of components of its regulon. In these conditions, the mRNA levels of the genes of the nonreductive pathway become increased, while those of FTR1 and FET3 remain low because of destabilization of the mRNAs. Consequently, the respective protein levels also remain low. Such mRNA destabilization is mediated by the general 5 –3 mRNA decay pathway and is independent of the RNA binding protein Cth2. Yeast cells are hypersensitive to peroxides in growth conditions where only the high-affinity reductive pathway is functional for iron assimilation. On the contrary, peroxide does not affect growth when iron uptake occurs exclusively through the non-reductive pathway. This reinforces the idea that upon oxidative stress S. cerevisiae cells redirect iron assimilation through the non-reductive pathway to minimize oxidative damage by the ferrous ions, which are formed during iron import through the Ftr1/Fet3 complexes.ca_ES
dc.language.isoengca_ES
dc.publisherJohn Wiley & Sonsca_ES
dc.publisherBlackwell Publishingca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1111/j.1365-2958.2011.07689.xca_ES
dc.relation.ispartofMolecular Microbiology, 2011, vol. 81, núm. 1, p. 232-248ca_ES
dc.rights(c) John Wiley & Sons, 2011ca_ES
dc.rights(c) Blackwell Publishing, 2011ca_ES
dc.titleThe oxidative stress response in yeast cells involves changes in the stability of Aft1 regulon mRNAsca_ES
dc.typearticleca_ES
dc.identifier.idgrec016840
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1111/j.1365-2958.2011.07689.x
dc.date.embargoEndDate2025-01-01


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