The oxidative stress response in yeast cells involves changes in the stability of Aft1 regulon mRNAs
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Saccharomyces cerevisiae can import iron through a high-affinity system consisting of the Ftr1/Fet3- mediated reductive pathway and the siderophoremediated non-reductive one. Expression of components of the high-affinity system is controlled by the Aft1 transcriptional factor. In this study we show that, upon oxidative stress, Aft1 is transitorily internalized into the nucleus, followed by transcription activation of components of its regulon. In these conditions, the mRNA levels of the genes of the nonreductive pathway become increased, while those of FTR1 and FET3 remain low because of destabilization of the mRNAs. Consequently, the respective protein levels also remain low. Such mRNA destabilization is mediated by the general 5 –3 mRNA decay pathway and is independent of the RNA binding protein Cth2. Yeast cells are hypersensitive to peroxides in growth conditions where only the high-affinity reductive pathway is functional for iron assimilation. On the contrary, peroxide does not affect growth when iron uptake occurs exclusively through the non-reductive pathway. This reinforces the idea that upon oxidative stress S. cerevisiae cells redirect iron assimilation through the non-reductive pathway to minimize oxidative damage by the ferrous ions, which are formed during iron import through the Ftr1/Fet3 complexes.
Is part ofMolecular Microbiology, 2011, vol. 81, núm. 1, p. 232-248
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Castells i Roca, Laia; García-Martínez, José; Moreno, Joaquín; Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma; Pérez-Ortín, José E. (Public Library of Science (PLoS), 2011)We have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25uC to 37uC. Using an established mathematical ...
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Castells i Roca, Laia; Pijuan Marquilles, Jordi; Ferrezuelo, Francisco; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique (Public Library of Science, 2016)Cth2 is an mRNA-binding protein that participates in remodeling yeast cell metabolism in iron starvation conditions by promoting decay of the targeted molecules, in order to avoid excess iron consumption. This study shows ...