| dc.contributor.author | Bellí i Martínez, Gemma | |
| dc.contributor.author | Polaina, Julio | |
| dc.contributor.author | Tamarit Sumalla, Jordi | |
| dc.contributor.author | Torre Ruiz, M. A. de la | |
| dc.contributor.author | Rodríguez Manzanaque, Maria Teresa | |
| dc.contributor.author | Ros Salvador, Joaquim | |
| dc.contributor.author | Herrero Perpiñán, Enrique | |
| dc.date.accessioned | 2015-07-07T10:20:56Z | |
| dc.date.issued | 2002 | |
| dc.identifier.issn | 0021-9258 | |
| dc.identifier.uri | http://hdl.handle.net/10459.1/48416 | |
| dc.description.abstract | Grx5 defines a family of yeast monothiol glutaredox-
ins that also includes Grx3 and Grx4. All three proteins
display significant sequence homology with proteins
found from bacteria to humans. Grx5 is involved in iron/
sulfur cluster assembly at the mitochondria, but the
function of Grx3 and Grx4 is unknown. Three-dimen-
sional modeling based on known dithiol glutaredoxin
structures predicted a thioredoxin fold structure for
Grx5. Positionally conserved amino acids in this glu-
taredoxin family were replaced in Grx5, and the effect
on the biological function of the protein has been tested.
For all changes studied, there was a correlation between
the effects on several different phenotypes: sensitivity
to oxidants, constitutive protein oxidation, ability for
respiratory growth, auxotrophy for a number of amino
acids, and iron accumulation. Cys60 and Gly61 are essen-
tial for Grx5 function, whereas other single or double
substitutions in the same region had no phenotypic ef-
fects. Gly115 and Gly116 could be important for the for-
mation of a glutathione cleft on the Grx5 surface, in
contrast to adjacent Cys117. Substitution of Phe50 alters
the -sheet in the thioredoxin fold structure and inhib-
its Grx5 function. None of the substitutions tested affect
the structure at a significant enough level to reduce
protein stability. | ca_ES |
| dc.language.iso | eng | ca_ES |
| dc.publisher | The American Society for Biochemistry and Molecular Biology | ca_ES |
| dc.relation.isformatof | Reproducció del document publicat a https://doi.org/10.1074/jbc.M201688200 | ca_ES |
| dc.relation.ispartof | The Journal of Biological Chemistry, 2002, vol. 277, núm 40, p. 37590-37596 | ca_ES |
| dc.rights | (c) The American Society for Biochemistry and Molecular Biology, 2002 | ca_ES |
| dc.title | Structure-function analysis of yeast Grx5 monothiol glutaredoxin defines essential amino acids for the function of the protein | ca_ES |
| dc.type | article | ca_ES |
| dc.identifier.idgrec | 004202 | |
| dc.type.version | publishedVersion | ca_ES |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_ES |
| dc.identifier.doi | https://doi.org/10.1074/jbc.M201688200 | |
