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dc.contributor.authorBellí i Martínez, Gemma
dc.contributor.authorPolaina, Julio
dc.contributor.authorTamarit Sumalla, Jordi
dc.contributor.authorTorre Ruiz, M. A. de la
dc.contributor.authorRodríguez Manzanaque, Maria Teresa
dc.contributor.authorRos Salvador, Joaquim
dc.contributor.authorHerrero Perpiñán, Enrique
dc.date.accessioned2015-07-07T10:20:56Z
dc.date.issued2002
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10459.1/48416
dc.description.abstractGrx5 defines a family of yeast monothiol glutaredox- ins that also includes Grx3 and Grx4. All three proteins display significant sequence homology with proteins found from bacteria to humans. Grx5 is involved in iron/ sulfur cluster assembly at the mitochondria, but the function of Grx3 and Grx4 is unknown. Three-dimen- sional modeling based on known dithiol glutaredoxin structures predicted a thioredoxin fold structure for Grx5. Positionally conserved amino acids in this glu- taredoxin family were replaced in Grx5, and the effect on the biological function of the protein has been tested. For all changes studied, there was a correlation between the effects on several different phenotypes: sensitivity to oxidants, constitutive protein oxidation, ability for respiratory growth, auxotrophy for a number of amino acids, and iron accumulation. Cys60 and Gly61 are essen- tial for Grx5 function, whereas other single or double substitutions in the same region had no phenotypic ef- fects. Gly115 and Gly116 could be important for the for- mation of a glutathione cleft on the Grx5 surface, in contrast to adjacent Cys117. Substitution of Phe50 alters the ␤-sheet in the thioredoxin fold structure and inhib- its Grx5 function. None of the substitutions tested affect the structure at a significant enough level to reduce protein stability.ca_ES
dc.language.isoengca_ES
dc.publisherThe American Society for Biochemistry and Molecular Biologyca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1074/jbc.M201688200ca_ES
dc.relation.ispartofThe Journal of Biological Chemistry, 2002, vol. 277, núm 40, p. 37590-37596ca_ES
dc.rights(c) The American Society for Biochemistry and Molecular Biology, 2002ca_ES
dc.titleStructure-function analysis of yeast Grx5 monothiol glutaredoxin defines essential amino acids for the function of the proteinca_ES
dc.typearticleca_ES
dc.identifier.idgrec004202
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1074/jbc.M201688200


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