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dc.contributor.authorMolina Navarro, Maria Micaela
dc.contributor.authorCastells i Roca, Laia
dc.contributor.authorBellí i Martínez, Gemma
dc.contributor.authorGarcía-Martínez, José
dc.contributor.authorMarín-Navarro, Julia
dc.contributor.authorMoreno, Joaquín
dc.contributor.authorPérez-Ortín, José E.
dc.contributor.authorHerrero Perpiñán, Enrique
dc.date.accessioned2015-07-07T08:50:50Z
dc.date.issued2008
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10459.1/48411
dc.description.abstractThe oxidative stress response in Saccharomyces cerevisiae has been analyzed by parallel determination of mRNA levels and transcription rates for the entire genome. A mathematical algorithm has been adapted for a dynamic situation such as the response to stress, to calculate theoretical mRNA decay rates from the experimental data. Yeast genes have been grouped into 25 clusters according to mRNA level and transcription rate kinetics, and average mRNA decay rates have been calculated for each cluster. In most of the genes, changes in one or both experimentally determined parameters occur during the stress response. 24% of the genes are transcriptionally induced without an increase inmRNAlevels. The lack of parallelism between the evolution of the mRNA amount and transcription rate predicts changes inmRNAstability during stress. Genes for ribosomal proteins and rRNA processing enzymes are abundant among those whose mRNAs are predicted to destabilize. The number of genes whose mRNAs are predicted to stabilize is lower, although some protein folding or proteasomal genes are among the latter. We have confirmed the mathematical predictions for several genes pertaining to different clusters by experimentally determining mRNA decay rates using the regulatable tetO promoter in transcriptional expression conditions not affected by the oxidative stress. This study indicates that the oxidative stress response in yeast cells is not only conditioned by gene transcription but also by the mRNA decay dynamics and that this complex response may be particularly relevant to explain the temporary down-regulation of protein synthesis occurring during stress.ca_ES
dc.language.isoengca_ES
dc.publisherThe American Society for Biochemistry and Molecular Biologyca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1074/jbc.M800295200ca_ES
dc.relation.ispartofThe Journal of Biological Chemistry, 2008, vol. 283, núm. 26, p. 17908-17918ca_ES
dc.rights(c) American Society for Biochemistry and Molecular Biology, 2008ca_ES
dc.titleComprehensive transcriptional analysis of the oxidative response in yeastca_ES
dc.typearticleca_ES
dc.identifier.idgrec012636
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1074/jbc.M800295200


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