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dc.contributor.authorBandyopadhyay, Sibali
dc.contributor.authorGama, Filipe
dc.contributor.authorMolina Navarro, Maria Micaela
dc.contributor.authorGualberto, José Manuel
dc.contributor.authorClaxton, Ronald
dc.contributor.authorHuynh, Boi Hanh
dc.contributor.authorHerrero Perpiñán, Enrique
dc.contributor.authorJacquot, Jean Pierre
dc.contributor.authorJohnson, Michael K.
dc.contributor.authorNaik, Sunil G.
dc.contributor.authorRouhier, Nicolas
dc.date.accessioned2015-07-07T08:33:36Z
dc.date.issued2008
dc.identifier.issn0261-4189
dc.identifier.urihttp://hdl.handle.net/10459.1/48407
dc.description.abstractGlutaredoxins (Grxs) are small oxidoreductases that reduce disulphide bonds or protein-glutathione mixed disulphides. More than 30 distinct grx genes are expressed in higher plants, but little is currently known concerning their functional diversity. This study presents biochemical and spectroscopic evidence for incorporation of a [2Fe–2S] cluster in two heterologously expressed chloroplastic Grxs, GrxS14 and GrxS16, and in vitro cysteine desulphurase-mediated assembly of an identical [2Fe–2S] cluster in apo-GrxS14. These Grxs possess the same monothiol CGFS active site as yeast Grx5 and both were able to complement a yeast grx5 mutant defective in Fe–S cluster assembly. In vitro kinetic studies monitored by CD spectroscopy indicate that [2Fe–2S] clusters on GrxS14 are rapidly and quantitatively transferred to apo chloroplast ferredoxin. These data demonstrate that chloroplast CGFS Grxs have the potential to function as scaffold proteins for the assembly of [2Fe–2S] clusters that can be transferred intact to physiologically relevant acceptor proteins. Alternatively, they may function in the storage and/or delivery of preformed Fe–S clusters or in the regulation of the chloroplastic Fe–S cluster assembly machinery.ca_ES
dc.language.isoengca_ES
dc.publisherEMBO Pressca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1038/ emboj.2008.50ca_ES
dc.relation.ispartofThe EMBO Journal, 2008, vol. 27, núm. 7, p. 1122-1133ca_ES
dc.rights(c) European Molecular Biology Organization, 2008ca_ES
dc.subjectchloroplastca_ES
dc.subjectglutaredoxinca_ES
dc.subjectiron-sulphur proteinca_ES
dc.titleChloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe–2S] clustersca_ES
dc.typearticleca_ES
dc.identifier.idgrec012129
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1038/emboj.2008.50
dc.date.embargoEndDate10000-01-01


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