Show simple item record

dc.contributor.authorBurón Moles, Gemma
dc.contributor.authorWisniewski, Michae
dc.contributor.authorViñas Almenar, Inmaculada
dc.contributor.authorTeixidó i Espasa, Neus
dc.contributor.authorUsall i Rodié, Josep
dc.contributor.authorDroby, Samir
dc.contributor.authorTorres Sanchis, Rosario
dc.date.accessioned2015-06-22T16:46:53Z
dc.date.issued2015
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10459.1/48372
dc.description.abstractApples are subjected to both abiotic and biotic stresses during the postharvest period, which lead to large economic losses worldwide. To obtain biochemical insights into apple defense response, we monitored the protein abundance changes (proteome), as well as the protein carbonyls (oxi-proteome) formed by reactive oxygen species (ROS) in"Golden Smoothee" apple in response to wounding, Penicillium expansum (host) and Penicillium digitatum (non-host) pathogens with select transcriptional studies. To examine the biological relevance of the results, we described quantitative and oxidative protein changes into the gene ontology functional categories, as well as into de KEGG pathways. We identified 26 proteins that differentially changed in abundance in response to wounding, P. expansum or P. digitatum infection. While these changes showed some similarities between the apple responses and abiotic and biotic stresses, Mal d 1.03A case, other proteins as Mal d 1.03E and EF-Tu were specifically induced in response to P. digitatum infection. Using a protein carbonyl detection method based on fluorescent Bodipy, we detected and identified 27 oxidized proteins as sensitive ROS targets. These ROS target proteins were related to metabolism processes, suggesting that this process plays a leading role in apple fruit defense response against abiotic and biotic stresses. ACC oxidase and two glutamine synthetases showed the highest protein oxidation level in response to P. digitatum infection. Documenting changes in the proteome and, specifically in oxi-proteome of apple can provide information that can be used to better understand how impaired protein functions may affect apple defense mechanisms. Possible mechanisms by which these modified proteins are involved in fruit defense response are discussed.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.jprot.2014.11.007
dc.relation.ispartofJournal of Proteomics, 2015, núm. 114, p. 136-151
dc.rights(c) Elsevier, 2015
dc.subjectMalus x domestica
dc.subjectGreen mold
dc.subjectBlue mold
dc.subjectprotein oxidation
dc.subjectRT-qPCR
dc.subject.classificationPomes
dc.subject.classificationTecnologia postcollita
dc.subject.classificationProteïnes dels aliments
dc.subject.otherApples
dc.subject.otherPostharvest technology
dc.subject.otherProtein content of food
dc.titleCharacterizing the proteome and oxi-proteome of apple in response to a compatible (Penicillium expansum) and a non-host (P. digitatum) pathogen.
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2015-06-22T16:46:53Z
dc.identifier.idgrec022561
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.identifier.doihttps://doi.org/10.1016/j.jprot.2014.11.007
dc.date.embargoEndDate2025-01-01


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record