Characterizing the proteome and oxi-proteome of apple in response to a compatible (Penicillium expansum) and a non-host (P. digitatum) pathogen.
Torres Sanchis, Rosario
MetadataShow full item record
Apples are subjected to both abiotic and biotic stresses during the postharvest period, which lead to large economic losses worldwide. To obtain biochemical insights into apple defense response, we monitored the protein abundance changes (proteome), as well as the protein carbonyls (oxi-proteome) formed
by reactive oxygen species (ROS) in"Golden Smoothee" apple in response to wounding, Penicillium expansum (host) and Penicillium digitatum (non-host) pathogens with select transcriptional studies. To examine the biological relevance of the results, we described quantitative and oxidative protein changes into the gene ontology functional categories, as well as into de KEGG pathways. We identified 26 proteins that differentially changed in abundance in response to wounding, P. expansum or P. digitatum infection. While these changes showed some similarities between the apple responses and abiotic and biotic stresses, Mal d 1.03A case, other proteins as Mal d 1.03E and EF-Tu were specifically induced in response to P. digitatum infection. Using a protein carbonyl detection method based on fluorescent Bodipy, we detected and identified 27 oxidized proteins as sensitive ROS targets. These ROS target proteins were related to metabolism processes, suggesting that this process plays a leading role in apple fruit defense response against abiotic and biotic stresses. ACC oxidase and two glutamine synthetases showed the highest protein oxidation level in response to P. digitatum infection. Documenting changes in the proteome and, specifically in oxi-proteome of apple can provide information that can be used to better understand how impaired protein functions may affect apple defense mechanisms. Possible mechanisms by which these modified proteins are involved in fruit defense response are discussed.
Is part ofJournal of Proteomics, 2015, núm. 114, p. 136-151
Showing items related by title, author, creator and subject.
Proteomic and oxi-proteomic response of apple to a compatible (P. expansum) and a non-host (P. digitatum) pathogen Burón Moles, Gemma; Wisniewski, M.; Viñas Almenar, Inmaculada; Teixidó i Espasa, Neus; Usall i Rodié, Josep; Droby, Samir; Torres, R. (ISHS, 2016)Despite the current use of chemical fungicides, Penicillium expansum still is one of the most devastating pathogens of pome fruit. In particular, P. expansum enters tissues through wounds causing large economic losses ...
Differential apple transcriptomic responses to Penicillium expansum (pathogen) and Penicillium digitatum (non-host pathogen) infection Vilanova, Laura; Wisniewski, M.; Norelli, J.; Viñas Almenar, Inmaculada; Torres, R.; Usall i Rodié, Josep; Phillips, J.; Droby, Samir; Teixidó i Espasa, Neus (ISHS, 2016)Penicillium expansum is the causal agent of blue mould of pome fruits and is responsible for important economical losses during postharvest handling in all producing countries. Although control of this pathogen can be ...
Increasing maturity reduces wound response and lignification processes against Penicillium expansum (pathogen) and P. digitatum (non-host pathogen) infection in apples Vilanova, Laura; Viñas Almenar, Inmaculada; Torres Sanchis, Rosario; Usall i Rodié, Josep; Burón Moles, Gemma; Teixidó i Espasa, Neus (Elsevier, 2014)Penicillium expansum is the main postharvest pathogen of pome fruit and is a necrotrophic fungus that requires wounds to infect the fruit. Therefore, injuries caused during harvest and postharvest handling provide an optimal ...