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dc.contributor.authorVarano della Vergiliana, Julius F.
dc.contributor.authorLansley, Sally M.
dc.contributor.authorPorcel Pérez, José Manuel
dc.contributor.authorBielsa Martín, Silvia
dc.contributor.authorBrown, Jeremy S.
dc.contributor.authorCreaney, Jenette
dc.contributor.authorTemple, Suzanna E. L.
dc.contributor.authorWaterer, Grant W.
dc.contributor.authorGary Lee, Y. C.
dc.description.abstractHeat shock protein 70 (HSP70) has been implicated in infection-related processes and has been found in body fluids during infection. This study aimed to determine whether pleural mesothelial cells release HSP70 in response to bacterial infection in vitro and in mouse models of serosal infection. In addition, the in vitro cytokine effects of the HSP70 isoform, Hsp72, on mesothelial cells were examined. Further, Hsp72 was measured in human pleural effusions and levels compared between non-infectious and infectious patients to determine the diagnostic accuracy of pleural fluid Hsp72 compared to traditional pleural fluid parameters. We showed that mesothelial release of Hsp72 was significantly raised when cells were treated with live and heat-killed Streptococcus pneumoniae. In mice, intraperitoneal injection of S. pneumoniae stimulated a 2-fold increase in Hsp72 levels in peritoneal lavage (p,0.01). Extracellular Hsp72 did not induce or inhibit mediator release from cultured mesothelial cells. Hsp72 levels were significantly higher in effusions of infectious origin compared to non-infectious effusions (p,0.05). The data establish that pleural mesothelial cells can release Hsp72 in response to bacterial infection and levels are raised in infectious pleural effusions. The biological role of HSP70 in pleural infection warrants exploration.ca_ES
dc.publisherPublic Library of Scienceca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofPLoS One, 2013, vol. 8, núm. 5, e63873ca_ES
dc.rightscc-by, (c) Varano della Vergiliana et al., 2013ca_ES
dc.titleBacterial infection elicits heat shock protein 72 release from pleural mesothelial cellsca_ES

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cc-by, (c) Varano della Vergiliana et al., 2013
Except where otherwise noted, this item's license is described as cc-by, (c) Varano della Vergiliana et al., 2013