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dc.contributor.authorBergadà Bertran, Laura
dc.contributor.authorYeramian Hakim, Andree
dc.contributor.authorSorolla Bardají, Anabel
dc.contributor.authorMatias-Guiu, Xavier
dc.contributor.authorDolcet Roca, Xavier
dc.date.accessioned2015-06-09T09:46:09Z
dc.date.available2015-06-09T09:46:09Z
dc.date.issued2014
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10459.1/48326
dc.description.abstractWe have recently demonstrated that histone deacetylase inhibitor, Vorinostat, applied as a single therapy or in combination with caspase-8 downregulation exhibits high anti-tumoral activity on endometrial carcinoma cell lines. In the present study, we have assessed the signalling processes underlying anti-tumoral effects of Vorinostat. Increasing evidence suggests that reactive oxygen species are responsible for histone deacetylase inhibitor-induced cell killing. We have found that Vorinostat induces formation of reactive oxygen species and DNA damage. To investigate the role of oxidative stress as anti-neoplastic mechanism, we have evaluated the effects of different antioxidants (Bha, Nac and Tiron) on endometrial carcinoma cell line Ishikawa treated with Vorinostat. We show that Bha, Nac and Tiron markedly inhibited the cytotoxic effects of Vorinostat, increasing cell viability in vitro. We found that all three antioxidants did not inhibited accumulation of acetyl Histone H4, so that antioxidants did not inhibit Vorinostat activity. Finally, we have evaluated the effects of antioxidants on anti-tumoral activity of Vorinostat as monotherapy or in combination with caspase-8 downregulation in vivo. Interestingly, antioxidants blocked the reduction of tumour growth caused by Vorinostat, but they were unable to inhibit anti-tumoral activity of Vorinostat plus caspase-8 inhibition.ca_ES
dc.language.isoengca_ES
dc.publisherPublic Library of Scienceca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1371/journal.pone.0092764ca_ES
dc.relation.ispartofPLoS One, 2014, vol. 9, núm. 3, e92764ca_ES
dc.rightscc-by, (c) Bergadà et al., 2014ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.titleAntioxidants impair anti-tumoral effects of Vorinostat, but not anti-neoplastic effects of Vorinostat and caspase-8 downregulationca_ES
dc.typearticleca_ES
dc.identifier.idgrec021110
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0092764


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cc-by, (c) Bergadà et al., 2014
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