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dc.contributor.authorBurón Moles, Gemma
dc.contributor.authorLópez-Pérez, M.
dc.contributor.authorGonzález-Candelas, Luis
dc.contributor.authorViñas Almenar, Inmaculada
dc.contributor.authorTeixidó i Espasa, Neus
dc.contributor.authorUsall i Rodié, Josep
dc.contributor.authorTorres Sanchis, Rosario
dc.date.accessioned2015-05-28T17:03:09Z
dc.date.issued2012
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/10459.1/48276
dc.description.abstractPenicillium digitatum and Penicillium expansum are responsible for green and blue molds in citrus and pome fruits, respectively, which result in major monetary losses worldwide. In order to study their infection process in fruits, we successfully introduced a green fluorescent protein (GFP) encoding gene into wild type P. digitatum and P. expansum isolates, using Agrobacterium tumefaciens-mediated transformation (ATMT), with hygromycin B resistance as the selectable marker. To our knowledge, this is the first report describing the transformation of these two important postharvest pathogens with GFP and the use of transformed strains to study compatible and non-host pathogen interactions. Transformation did not affect the pathogenicity or the ecophysiology of either species compared to their respective wild type strains. The GFP-tagged strains were used for in situ analysis of compatible and non-host pathogen interactions on oranges and apples. Knowledge of the infection process of apples and oranges by these pathogens will facilitate the design of novel strategies to control these postharvest diseases and the use of the GFP-tagged strains will help to determine the response of P. digitatum and P. expansum on/in plant surface and tissues to different postharvest treatments.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.ijfoodmicro.2012.10.005
dc.relation.ispartofInternational Journal of Food Microbiology, 2012, núm. 160, p. 162-170
dc.rights(c) Elsevier, 2012
dc.subjectATMT
dc.subjectPopulation monitoring
dc.subjectEcophysiology
dc.subjectPathogenicity
dc.subjectApple and orange
dc.subject.classificationCítrics
dc.subject.classificationPomes
dc.subject.classificationEcofisiologia vegetal
dc.subject.classificationMalalties i plagues postcollita
dc.subject.otherCitrus
dc.subject.otherApples
dc.subject.otherPlant ecophysiology
dc.titleUse of GFP-tagged strains of Penicillium digitatum and Penicillium expansum to study host-pathogen interactions in oranges and apples.
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2015-05-28T17:03:10Z
dc.identifier.idgrec022566
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.identifier.doihttps://doi.org/10.1016/j.ijfoodmicro.2012.10.005
dc.date.embargoEndDate2025-01-01


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