Use of GFP-tagged strains of Penicillium digitatum and Penicillium expansum to study host-pathogen interactions in oranges and apples.
Torres Sanchis, Rosario
MetadataShow full item record
Penicillium digitatum and Penicillium expansum are responsible for green and blue molds in citrus and pome fruits, respectively, which result in major monetary losses worldwide. In order to study their infection process in fruits, we successfully introduced a green fluorescent protein (GFP) encoding gene into wild type P. digitatum and P. expansum isolates, using Agrobacterium tumefaciens-mediated transformation (ATMT), with hygromycin B resistance as the selectable marker. To our knowledge, this is the first report describing the transformation of these two important postharvest pathogens with GFP and the use of transformed strains to study compatible and non-host pathogen interactions. Transformation did not affect the pathogenicity or the ecophysiology of either species compared to their respective wild type strains. The GFP-tagged strains were used for in situ analysis of compatible and non-host pathogen interactions on oranges and apples. Knowledge of the infection process of apples and oranges by these pathogens will facilitate the design of novel strategies to control these postharvest diseases and the use of the GFP-tagged strains will help to determine the response of P. digitatum and P. expansum on/in plant surface and tissues to different postharvest treatments.
Is part ofInternational Journal of Food Microbiology, 2012, núm. 160, p. 162-170
European research projects
Showing items related by title, author, creator and subject.
Wound response in orange as a resistance mechanism against Penicillium digitatum (pathogen) and P. expansum (non-host pathogen) Vilanova, Laura; Torres Sanchis, Rosario; Viñas Almenar, Inmaculada; González-Candelas, Luis; Usall i Rodié, Josep; Fiori, S.; Solsona Aixalà, Cristina; Teixidó i Espasa, Neus (Elsevier, 2013)Penicillium digitatum is the most devastating postharvest pathogen of citrus. In addition, Penicillium expansum is the main pathogen of pome fruit, although recent studies have demonstrated its ability to infect oranges ...
Effect of fruit maturity stage of orange on the wound response to Penicillium digitatum (pathogen) and P. expansum (non-host pathogen) Vilanova, Laura; Torres Sanchis, Rosario; Usall i Rodié, Josep; Teixidó i Espasa, Neus; González-Candelas, Luis; Viñas Almenar, Inmaculada; Fiori, S. (International Society for Horticultural Science, 2014)Penicillium digitatum and P. expansum are postharvest necrotrophic fungi that require wounds to infect the fruit. Therefore, injuries caused during harvesting and postharvest handling provide an optimal locus for infection. ...
Differential contribution of the two major polygalacturonases from Penicillium digitatum to virulence towards citrus fruit Vilanova Torren, Laura; López-Pérez, M.; Ballester, Ana-Rosa; Teixidó i Espasa, Neus; Usall i Rodié, Josep; Lara Ayala, Isabel; Viñas Almenar, Inmaculada; Torres Sanchis, Rosario; González-Candelas, Luis (Elsevier, 2018-10)The fungus Penicillium digitatum is the causal agent of the citrus green mould, the major postharvest diseases of citrus fruit. Lesions on the surface of infected fruits first appear as soft areas around the inoculation ...