Protein Kinase KIS Localizes to RNA Granules and Enhances Local Translation

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Cambray Carner, SerafíCambray Carner, Serafí - ORCID ID
Pedraza González, NeusPedraza González, Neus - ORCID ID
Rafel i Borrell, MartaRafel i Borrell, Marta - ORCID ID
Garí Marsol, EloiGarí Marsol, Eloi - ORCID ID
Aldea, Martí
Gallego, CarmeGallego, Carme - ORCID ID
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The regulation of mRNA transport is a fundamental process for cytoplasmic sorting of transcripts and spatially controlled translational derepression once properly localized. There is growing evidence that translation is locally modulated as a result of specific synaptic inputs. However, the underlying molecular mechanisms that regulate this translational process are just emerging. We show that KIS, a serine/threonine kinase functionally related to microtubule dynamics and axon development, interacts with three proteins found in RNA granules: KIF3A, NonO, and eEF1A. KIS localizes to RNA granules and colocalizes with the KIF3A kinesin and the β-actin mRNA in cultured cortical neurons. In addition, KIS is found associated with KIF3A and 10 RNP-transported mRNAs in brain extracts. The results of knockdown experiments indicate that KIS is required for normal neurite outgrowth. More important, the kinase activity of KIS stimulates 3' untranslated region-dependent local translation in neuritic projections. We propose that KIS is a component of the molecular device that modulates translation in RNA-transporting granules as a result of local signals.
Journal or Serie
Molecular and cellular biology, 2009, vol. 29, núm. 3, p. 726-735