Protein Kinase KIS Localizes to RNA Granules and Enhances Local Translation
Loading...
Files
Date
2009
Authors
Cambray Carner, Serafí
Pedraza González, Neus
Rafel i Borrell, Marta
Garí Marsol, Eloi
Aldea, Martí
Gallego, Carme
Other authors
Impact
Export
Share
Journal Title
Journal ISSN
Volume Title
Abstract
The regulation of mRNA transport is a fundamental process for cytoplasmic sorting of transcripts and
spatially controlled translational derepression once properly localized. There is growing evidence that translation
is locally modulated as a result of specific synaptic inputs. However, the underlying molecular mechanisms
that regulate this translational process are just emerging. We show that KIS, a serine/threonine kinase
functionally related to microtubule dynamics and axon development, interacts with three proteins found in
RNA granules: KIF3A, NonO, and eEF1A. KIS localizes to RNA granules and colocalizes with the KIF3A
kinesin and the β-actin mRNA in cultured cortical neurons. In addition, KIS is found associated with KIF3A
and 10 RNP-transported mRNAs in brain extracts. The results of knockdown experiments indicate that KIS
is required for normal neurite outgrowth. More important, the kinase activity of KIS stimulates 3' untranslated
region-dependent local translation in neuritic projections. We propose that KIS is a component of the
molecular device that modulates translation in RNA-transporting granules as a result of local signals.
Citation
Journal or Serie
Molecular and cellular biology, 2009, vol. 29, núm. 3, p. 726-735