Protein Kinase KIS Localizes to RNA Granules and Enhances Local Translation

Thumbnail
View/Open
Issue date
2009
Author
Cambray Carner, Serafí
Pedraza González, Neus
Rafel i Borrell, Marta
Garí Marsol, Eloi
Aldea, Martí
Gallego, Carme
Suggested citation
Cambray Carner, Serafí; Pedraza González, Neus; Rafel i Borrell, Marta; Garí Marsol, Eloi; Aldea, Martí; Gallego, Carme; . (2009) . Protein Kinase KIS Localizes to RNA Granules and Enhances Local Translation. Molecular and cellular biology, 2009, vol. 29, núm. 3, p. 726-735. https://doi.org/10.1128/MCB.01180-08.
Impact

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
The regulation of mRNA transport is a fundamental process for cytoplasmic sorting of transcripts and spatially controlled translational derepression once properly localized. There is growing evidence that translation is locally modulated as a result of specific synaptic inputs. However, the underlying molecular mechanisms that regulate this translational process are just emerging. We show that KIS, a serine/threonine kinase functionally related to microtubule dynamics and axon development, interacts with three proteins found in RNA granules: KIF3A, NonO, and eEF1A. KIS localizes to RNA granules and colocalizes with the KIF3A kinesin and the β-actin mRNA in cultured cortical neurons. In addition, KIS is found associated with KIF3A and 10 RNP-transported mRNAs in brain extracts. The results of knockdown experiments indicate that KIS is required for normal neurite outgrowth. More important, the kinase activity of KIS stimulates 3' untranslated region-dependent local translation in neuritic projections. We propose that KIS is a component of the molecular device that modulates translation in RNA-transporting granules as a result of local signals.
URI
http://hdl.handle.net/10459.1/48183
DOI
https://doi.org/10.1128/MCB.01180-08
Is part of
Molecular and cellular biology, 2009, vol. 29, núm. 3, p. 726-735
Collections