Show simple item record

dc.contributor.authorMachín, Félix
dc.contributor.authorTorres Rosell, Jordi
dc.contributor.authorPiccoli, Giacomo De
dc.contributor.authorCarballo, Jesús A.
dc.contributor.authorCha, Rita S.
dc.contributor.authorJarmuz, Adam
dc.contributor.authorAragón, Luis
dc.description.abstractMitotic disjunction of the repetitive ribosomal DNA (rDNA) involves specialized segregation mechanisms dependent on the conserved phosphatase Cdc14. The reason behind this requirement is unknown. We show that rDNA segregation requires Cdc14 partly because of its physical length but most importantly because a fraction of ribosomal RNA (rRNA) genes are transcribed at very high rates. We show that cells cannot segregate rDNA without Cdc14 unless they undergo genetic rearrangements that reduce rDNA copy number. We then demonstrate that cells with normal length rDNA arrays can segregate rDNA in the absence of Cdc14 as long as rRNA genes are not transcribed. In addition, our study uncovers an unexpected role for the replication barrier protein Fob1 in rDNA segregation that is independent of Cdc14. These fi ndings demonstrate that highly transcribed loci can cause chromosome nondisjunction.ca_ES
dc.publisherThe Rockefeller University Pressca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofJournal of Cell Biology, 2006, vol. 173, núm. 6, p. 883-903ca_ES
dc.rightsby-nc-sa (c) Machín et al., 2006ca_ES
dc.titleTranscription of ribosomal genes can cause nondisjunctionca_ES

Files in this item


This item appears in the following Collection(s)

Show simple item record

by-nc-sa (c) Machín et al., 2006
Except where otherwise noted, this item's license is described as by-nc-sa (c) Machín et al., 2006