Universitat de Lleida
    • English
    • català
    • español
  • español 
    • English
    • català
    • español
  • Iniciar sesión
Repositori Obert UdL
Ver ítem 
  •   Inicio
  • Recerca
  • Medicina Experimental
  • Articles publicats (Medicina Experimental)
  • Ver ítem
  •   Inicio
  • Recerca
  • Medicina Experimental
  • Articles publicats (Medicina Experimental)
  • Ver ítem
JavaScript is disabled for your browser. Some features of this site may not work without it.

BAX and BAK proteins are required for cyclin-dependent kinase inhibitory drugs to cause apoptosis

Thumbnail
Ver/Abrir
013097.pdf (9.166Mb)
Fecha de publicación
2008
Autor/a
Garrofé Ochoa, Xènia
Melero Fernández de Mera, Raquel M
Fernández Gómez, Francisco J.
Ribas i Fortuny, Judit
Jordán Bueso, Joaquín
Boix Torras, Jacint
Cita recomendada
Garrofé Ochoa, Xènia; Melero Fernández de Mera, Raquel M; Fernández Gómez, Francisco J.; Ribas i Fortuny, Judit; Jordán Bueso, Joaquín; Boix Torras, Jacint; . (2008) . BAX and BAK proteins are required for cyclin-dependent kinase inhibitory drugs to cause apoptosis. Molecular Cancer Therapeutics, 2008, vol. 7, num. 12, p. 3800-3806. https://doi.org/10.1158/1535-7163.MCT-08-0655.
Impacto


Logo de Web of Science    citaciones en Web of Science

Logo de Scopus    citaciones en Scopus

Logo de Google Académico  Google Académico
Compartir
Exportar a Mendeley
Metadatos
Mostrar el registro completo del ítem
Resumen
In previous reports we have shown in SH-SY5 cells that Olomoucine and Roscovitine, two inhibitory drugs of Cyclin Dependent Kinases, caused apoptosis independent of the extrinsic pathway. In this experimental paradigm, apoptosis was refractory to the protective effects of either Bcl-2 or Bcl-XL overexpression. We are now reporting the failure of Bcl-XL to prevent dell death was consistent with no effect on the kinetics of caspase activation and cytochrome c release. To further characterize this issue, we have discarded a direct effect of either Olomoucine or Roscovitine on mitochondrial permeability transition. Moreover, we have evidence that an intrinsic pathway took place in SH-SY5Y cells by demonstrating the mitochondrial translocation of a GFP-Bax construct upon transfection and treatment with CDK inhibitory drugs. Finally, we tested the effect of Olomoucine and Roscovitine on w.t., bax-/-, bak-/- and double bax-/-bak-/- MEFs. In w.t. MEFs, both drugs induced cell death by apoptosis in a dose-dependent manner. In bax-/-, bak-/- and, particularly, double bax-/-bak-/- MEFs, we observed the inhibition of apoptosis. In conclusion, Olomoucine and Roscovitine caused apoptosis through an intrinsic pathway with Bax and Bak proteins being involved.
URI
http://hdl.handle.net/10459.1/48087
DOI
https://doi.org/10.1158/1535-7163.MCT-08-0655
Es parte de
Molecular Cancer Therapeutics, 2008, vol. 7, num. 12, p. 3800-3806
Proyectos de investigación europeos
Colecciones
  • Articles publicats (IRBLleida) [755]
  • Articles publicats (Medicina Experimental) [246]
  • Articles publicats (Grup de Recerca en Neurobiologia Cel·lular) [19]

Publicaciones relacionadas

Showing items related by title, author, creator and subject.

  • Trancriptional modulation of apoptotis regulators by Roscovitine and related compounds 

    Garrofé Ochoa, Xènia; Cosialls, Ana M; Ribas i Fortuny, Judit; Gil, Joan; Boix Torras, Jacint (Springer Science Business Media, 2011)
    Chemical inhibitors of cyclin-dependent kinase (CDK), like roscovitine, are promising drugs in thecontext of new cancer therapies. Roscovitine and related compounds, like seliciclib and olomoucine, are effective inducers ...
  • 7-Bromoindirubin-3'-oxime induces caspase-independent cell death 

    Ribas i Fortuny, Judit; Bettayeb, Karima; Ferandin, Yoan; Knockaert, Marie; Garrofé Ochoa, Xènia; Totzke, Frank; Schächtele, Christoph; Mester, Jan; Polychronopoulos, Panagiotis; Magiatis, Prokopios; Skaltsounis, Alexios-Leandros; Boix Torras, Jacint; Meijer, Laurent (Nature Publishing Group, 2006)
    Indirubin, an isomer of indigo, is a reported inhibitor of cyclin-dependent kinases (CDKs) and glycogen synthase kinase-3 (GSK-3) as well as an agonist of the aryl hydrocarbon receptor (AhR). Indirubin is the active ...
  • 7-Bromoindirubin-3'-oxime uncovers a serine protease-mediated paradigm of necrotic cell death 

    Ribas i Fortuny, Judit; Yuste Mateos, Víctor J. (Víctor José); Garrofé Ochoa, Xènia; Meijer, Laurent; Esquerda Colell, Josep; Boix Torras, Jacint (Elsevier, 2008)
    The new 7-bromoindirubin-3′-oxime (7BIO) compound induces caspase-independent cell death in all cell lines tested to date. Irrespective of the cell line, a 25 μM treatment for 24 h is lethal for the entire cell population. ...

Contacto | Sugerencias | Aviso legal
© 2021 BiD. Universitat de Lleida
Metadatos sujetos a 
 

 

Explorar

Todo el repositorioComunidades y coleccionesPor fecha de publicaciónAutoresTítulosMateriasEsta colecciónPor fecha de publicaciónAutoresTítulosMaterias

Estadísticas

Ver Estadísticas de uso

De interés

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contacto | Sugerencias | Aviso legal
© 2021 BiD. Universitat de Lleida
Metadatos sujetos a