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BAX and BAK proteins are required for cyclin-dependent kinase inhibitory drugs to cause apoptosis

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Issue date
2008
Author
Garrofé Ochoa, Xènia
Melero Fernández de Mera, Raquel M
Fernández Gómez, Francisco J.
Ribas i Fortuny, Judit
Jordán Bueso, Joaquín
Boix Torras, Jacint
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Garrofé Ochoa, Xènia; Melero Fernández de Mera, Raquel M; Fernández Gómez, Francisco J.; Ribas i Fortuny, Judit; Jordán Bueso, Joaquín; Boix Torras, Jacint; . (2008) . BAX and BAK proteins are required for cyclin-dependent kinase inhibitory drugs to cause apoptosis. Molecular Cancer Therapeutics, 2008, vol. 7, num. 12, p. 3800-3806. https://doi.org/10.1158/1535-7163.MCT-08-0655.
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Abstract
In previous reports we have shown in SH-SY5 cells that Olomoucine and Roscovitine, two inhibitory drugs of Cyclin Dependent Kinases, caused apoptosis independent of the extrinsic pathway. In this experimental paradigm, apoptosis was refractory to the protective effects of either Bcl-2 or Bcl-XL overexpression. We are now reporting the failure of Bcl-XL to prevent dell death was consistent with no effect on the kinetics of caspase activation and cytochrome c release. To further characterize this issue, we have discarded a direct effect of either Olomoucine or Roscovitine on mitochondrial permeability transition. Moreover, we have evidence that an intrinsic pathway took place in SH-SY5Y cells by demonstrating the mitochondrial translocation of a GFP-Bax construct upon transfection and treatment with CDK inhibitory drugs. Finally, we tested the effect of Olomoucine and Roscovitine on w.t., bax-/-, bak-/- and double bax-/-bak-/- MEFs. In w.t. MEFs, both drugs induced cell death by apoptosis in a dose-dependent manner. In bax-/-, bak-/- and, particularly, double bax-/-bak-/- MEFs, we observed the inhibition of apoptosis. In conclusion, Olomoucine and Roscovitine caused apoptosis through an intrinsic pathway with Bax and Bak proteins being involved.
URI
http://hdl.handle.net/10459.1/48087
DOI
https://doi.org/10.1158/1535-7163.MCT-08-0655
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Molecular Cancer Therapeutics, 2008, vol. 7, num. 12, p. 3800-3806
European research projects
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  • Articles publicats (Medicina Experimental) [350]
  • Articles publicats (Grup de Recerca en Neurobiologia Cel·lular) [19]
  • Articles publicats (IRBLleida) [1167]

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