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dc.contributor.authorFeregrino-Perez AA
dc.contributor.authorPiñol Felis, Carme
dc.contributor.authorGómez Arbonés, Javier
dc.contributor.authorGuevara-González RG
dc.contributor.authorCampos-Vega R
dc.contributor.authorAcosta-Gallegos J
dc.contributor.authorLoarca-Piña G
dc.date.accessioned2015-03-18T16:42:03Z
dc.date.issued2014-06-21
dc.identifier.issn0921-9668
dc.identifier.urihttp://hdl.handle.net/10459.1/48071
dc.description.abstractWe have previously demonstrated that the non-digestible fraction (NDF) from common cooked beans (P. vulgaris L., cv Negro 8025) inhibits azoxymethane (AOM) induced colon cancer and influences the expression of genes involved in the induction of apoptosis and cell cycle arrest through the action of butyrate. The objective of this study was to identify cell cycle alterations and morphological changes induced by treatment with AOM and to examine the formation of colonic aberrant crypt foci (ACF) in male Sprague Dawley rats fed with these beans. Rats were fed control diets upon arrival and were randomly placed into four groups after one week of acclimatization: control, NDF (intragastric administration), NDF+AOM and AOM. Rats treated with NDF+AOM exhibited a significantly lower number of total colonic ACF with a notable increase in the number of cells present in the G1 phase (83.14 %); a decreased proliferation index was observed in the NDF+AOM group when compared to AOM group. NDF+AOM also displayed a higher number of apoptotic cells compared to AOM group. NDF of cooked common beans inhibited colon carcinogenesis at an early stage by inducing cell cycle arrest ofcolon cells and morphological changes linked to apoptosis, thus confirming previous results obtained with gene expression studies.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherSpringer
dc.relation.isformatofVersió postprint del document publicat a: 10.1007/s11130-014-0428-7
dc.relation.ispartofPlant Foods for Human Nutrition, 2014, vol. 69, num. 3, p. 248-254
dc.rights(c) Springer Science Business Media, 2014
dc.subjectNon-digestible fraction
dc.subjectPhaseolus vulgaris L.
dc.subjectFlow cytometry
dc.subjectApoptosis
dc.subjectAberrant crypt foci
dc.subject.classificationApoptosi
dc.subject.classificationCarcinogènesi
dc.subject.classificationCitometria de fluxe
dc.subject.otherApoptosis
dc.subject.otherCarcinogenesis
dc.subject.otherFlow cytometry
dc.titleA Non-digestible Fraction of the Common Bean (Phaseolus vulgaris L.) Induces Cell Cycle Arrest and Apoptosis During Early Carcinogenesis
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2015-03-18T16:42:03Z
dc.identifier.idgrec021378
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.identifier.doihttps://doi.org/10.1007/s11130-014-0428-7
dc.date.embargoEndDate10000-01-01


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