Browsing Institut de Recerca Biomèdica de Lleida (IRBLleida) by Issue Date
Now showing items 1-20 of 1085
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Analysis of U1snRNP-specific A protein cross-linked complexes
(Wiley, 1990)The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, ... -
Separation of rooster spermatogenic nuclei by means of centrifugal elutriation
(Wiley, 1993)By application of minor variations in the usual method of centrifugal elutriation, we have been able to separate a nuclear suspension from rooster testes. We have obtained a population of elongated or late spermatid ... -
Characterization of the Cell Death Process Induced by Staurosporine in Human Neuroblastoma Cell Lines
(Elsevier, 1997)Staurosporine is a potent and non-specific inhibitor of protein kinases. There is also evidence of staurosporine being a potent inducer of apoptosis. In several human neuroblastoma cell lines (SH-SY5Y, NB69, IMR-5 and ... -
Mitochondrial membrane peroxidizability index is inversely related to maximum life span in mammals
(Elsevier, 1998)The oxidative stress theory of aging predicts a low degree of fatty acid unsaturation in tissues of longevous animals, because membrane lipids increase their sensitivity to oxidative damage as a function of their unsaturation. ... -
Neonatal tumor necrosis factor alpha promotes diabetes in nonobese diabetic mice by CD154-independent antigen presentation to CD8(+) T cells
(Rockefeller University Press, 2000)Neonatal islet-specific expression of tumor necrosis factor (TNF)-α in nonobese diabetic mice promotes diabetes by provoking islet-infiltrating antigen-presenting cells to present islet peptides to autoreactive T cells. ... -
Oxidative stress promotes specific protein damage in Saccharomyces cerevisiae
(The American Society for Biochemistry and Molecular Biology, 2000)We have analyzed the proteins that are oxidatively damaged when Saccharomyces cerevisiae cells are ex- posed to stressing conditions. Carbonyl groups generated by hydrogen peroxide or menadione on proteins of aero- bically ... -
Isolation of a Candida albicans gene, tightly linked to URA3, coding for a putative transcription factor that suppresses a Saccharomyces cerevisiae aft1 mutation
(John Wiley & Sons, 2001)A pathogen such as C. albicans needs an ef®cient mechanism of iron uptake in an iron- restricted environment such as is the human body. A ferric-reductase activity regulated by iron and copper, and analogous to that in ... -
Osmotic stress causes a G1 cell cycle delay and downregulation of Cln3/Cdc28 activity in Saccharomyces cerevisiae
(Blackwell Science, 2001)Moderate hyperosmotic stress on Saccharomyces cerevisiae cells produces a temporary delay at the G1 stage of the cell cycle. This is accompanied by transitory downregulation of CLN1, CLN2 and CLB5 transcript levels, ... -
Mitochondrial Hsp60, resistance to oxidative stress, and the labile iron pool are closely connected in Saccharomyces cerevisiae
(The American Society for Biochemistry and Molecular Biology, 2002)In the present study, we have analyzed the role of the molecular chaperone Hsp60 in protection of Saccharo- myces cerevisiae against oxidative damage. We con- structed mutant strains in which the levels of Hsp60 protein, ... -
Regulation of the cell integrity pathway by rapamycin-sensitive TOR function in budding yeast
(The American Society for Biochemistry and Molecular Biology, 2002)The TOR (target of rapamycin) pathway controls cell growth in response to nutrient availability in eukary- otic cells. Inactivation of TOR function by rapamycin or nutrient exhaustion is accompanied by triggering various ... -
Structure-function analysis of yeast Grx5 monothiol glutaredoxin defines essential amino acids for the function of the protein
(The American Society for Biochemistry and Molecular Biology, 2002)Grx5 defines a family of yeast monothiol glutaredox- ins that also includes Grx3 and Grx4. All three proteins display significant sequence homology with proteins found from bacteria to humans. Grx5 is involved in ... -
Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae
(The American Society for Biochemistry and Molecular Biology, 2002)Maintenance of cellular integrity in Saccharomyces cerevisiae is carried out by the activation of the protein kinase C-mediated mitogen-activated protein kinase (PKC1-MAPK) pathway. Here we report that correct down-regulation ... -
Comparative genomics of yeast species: new insights into their biology
(Sociedad Española de Microbiología, 2003)The genomes of two hemiascomycetous yeasts (Saccharomyces cerevisiae and Candida albicans) and one archiascomycete (Schizosaccharomyces pombe) have been completely sequenced and the genes have been annotated. In addition, ... -
Biochemical characterization of yeast mitochondrial Grx5 monothiol glutaredoxin
(The American Society for Biochemistry and Molecular Biology, 2003)Grx5 is a yeast mitochondrial protein involved in iron- sulfur biogenesis that belongs to a recently described family of monothiolic glutaredoxin-like proteins. No member of this family has been biochemically charac- terized ... -
Prokaryotic and eukaryotic monothiol glutaredoxins are able to perform the functions of Grx5 in the biogenesis of Fe/S clusters in yeast mitochondria
(Elsevier, 2003)The Saccharomyces cerevisiae monothiol glutare- doxin Grx5 participates in the mitochondrial biogenesis of iron–sulfur clusters. Grx5 homologues exist in organisms from bacteria to humans. Chicken (cGRX5) and human ... -
Characterization of a Candida albicans gene encoding a putative transcriptional factor required for cell wall integrity
(Elsevier, 2003)After screening a Candida albicans genome database the product of an open reading frame (ORF) (CA2880) with 49% homology to the product of Saccharomyces cerevisiae YPL133c, a putative transcriptional factor, was identified. ... -
Cell differentiation, caspase inhibition and macromolecular synthesis blockage, but not Bcl-2 or Bcl-XL proteins, protect SH-SY5Y cells from apoptosis triggered by two cdk inhibitory drugs.
(Elsevier, 2004)Olomoucine and Roscovitine are two ATP-competing compounds described as specific inhibitors of cyclin-dependent kinases (CDK). Both drugs showed to induce apoptosis in SH-SY5Y, a neuroblastoma-derived cell line. In these ... -
Evolution and cellular function of monothiol glutaredoxins: involvement in iron-sulphur cluster assembly
(Wiley Interscience, 2004)A number of bacterial species, mostly proteobacteria, possess monothiol glutaredoxins homologous to the Saccharomyces cerevisiae mitochondrial protein Grx5, which is involved in iron–sulphur cluster synthesis. Phylogenetic ... -
Saccharomyces cerevisiae glutaredoxin 5-deficient cells subjected to continuous oxidizing conditions are affected in the expression of specific sets of genes
(The American Society for Biochemistry and Molecular Biology, 2004)The Saccharomyces cerevisiae GRX5 gene codes for a mitochondrial glutaredoxin involved in the synthesis of iron/sulfur clusters. Its absence prevents respiratory growth and causes the accumulation of iron inside cells and ... -
Relationships between big five personality factors and values
(Society for Personality Research, 2004)An analysis of principal components showed a robust structure of three factors formed by 25 of the 30 values studied. These factors were called Social Power, Order and Benevolence values. Those factors match with some ...