Browsing Institut de Recerca Biomèdica de Lleida (IRBLleida) by Author "Torre Ruiz, M. A. de la"
Now showing items 1-19 of 19
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A genome-wide transcriptional study reveals that iron deficiency inhibits the yeast TORC1 pathway
Romero, Antonia María; Ramos-Alonso, Lucía; Montellá-Manuel, Sandra; García-Martínez, José; Torre Ruiz, M. A. de la; Pérez-Ortín, José E.; Martínez-Pastor, María Teresa; Puig, Sergi (Elsevier, 2019-09)Iron is an essential micronutrient that participates as a cofactor in a broad range of metabolic processes including mitochondrial respiration, DNA replication, protein translation and lipid biosynthesis. Adaptation to ... -
Both human and soya bean ferritins highly improve the accumulation of bioavailable iron and contribute to extend the chronological life in budding yeast
Pujol Carrión, Núria; González-Alfonso, Alma; Puig, Sergi; Torre Ruiz, M. A. de la (Society for Applied Microbiology; John Wiley & Sons Ltd., 2021)Ferritin proteins have an enormous capacity to store iron in cells. In search for the best conditions to accumulate and store bioavailable iron, we made use of a double mutant null for the monothiol glutaredoxins GRX3 and ... -
Bulk autophagy induction and life extension is achieved when iron is the only limited nutrient in Saccharomyces cerevisiae
Montellá-Manuel, Sandra; Pujol Carrión, Núria; Mechoud, Mónica A.; Torre Ruiz, M. A. de la (Portland Press; Biochemical Society, 2021)We have investigated the effects that iron limitation provokes in Saccharomyces cerevisiae exponential cultures. We have demonstrated that one primary response is the induction of bulk autophagy mediated by TORC1. Coherently, ... -
Chronological and replicative life-span extension in Saccharomyces cerevisiae by increased dosage of alcohol dehydrogenase 1
Reverter Branchat, Gemma; Cabiscol Català, Elisa; Tamarit Sumalla, Jordi; Sorolla Bardají, Maria Alba; Torre Ruiz, M. A. de la; Ros Salvador, Joaquim (Microbiology Society, 2007)Alcohol dehydrogenase 1 (Adh1)p catalyses the conversion of acetaldehyde to ethanol, regenerating NAD+. In Saccharomyces cerevisiae, Adh1p is oxidatively modified during ageing and, consequently, its activity becomes ... -
Comparative genomics of yeast species: new insights into their biology
Herrero Perpiñán, Enrique; Torre Ruiz, M. A. de la; Valentín, Eulogio (Sociedad Española de Microbiología, 2003)The genomes of two hemiascomycetous yeasts (Saccharomyces cerevisiae and Candida albicans) and one archiascomycete (Schizosaccharomyces pombe) have been completely sequenced and the genes have been annotated. In addition, ... -
Coping with oxidative stress. The yeast model
Torre Ruiz, M. A. de la; Pujol Carrión, Núria; Sundaram, Venkatraghavan (Bentham Science Publishers, 2015-01-22)Saccharomyces cerevisiae is an optimal model to study stress responses for various reasons: i) budding yeast genome presents a high degree of homology with the human genome; ii) there are many proteins that show an elevated ... -
Glutaredoxins Grx4 and Grx3 of Saccharomyces cerevisiae Play a Role in Actin Dynamics through Their Trx Domains, Which Contributes to Oxidative Stress Resistance
Pujol Carrión, Núria; Torre Ruiz, M. A. de la (American Society for Microbiology, 2010)Grx3 and Grx4 are two monothiol glutaredoxins of Saccharomyces cerevisiae that have previously been characterized as regulators of Aft1 localization and therefore of iron homeostasis. In this study, we present data showing ... -
Interactions of GMP with human Glrx3 and with Saccharomyces cerevisiae Grx3 and Grx4 converge in the regulation of the Gcn2 pathway
Mechoud, Mónica A.; Pujol Carrión, Núria; Montellá-Manuel, Sandra; Torre Ruiz, M. A. de la (American Society for Microbiology, 2020-07-02)The human monothiol glutaredoxin Glrx3 (PICOT) is ubiquitously distributed in cytoplasm and nuclei in mammalian cells. Its overexpression has been associated with the development of several types of tumors, whereas its ... -
Mtl1 is required to activate general stress response through Tor1 and Ras2 inhibition under conditions of glucose starvation and oxidative stress
Petkova, Mima Ivanova; Pujol Carrión, Núria; Arroyo, Javier; Garcia-Cantalejo, J.; Torre Ruiz, M. A. de la (American Society for Biochemistry and Molecular Biology, 2010)Mtl1 is a member of the cell wall integrity (CWI) pathway of Saccharomyces cerevisiae, which functions as a cell wall sensor for oxidative stress. Genome-wide transcriptional analysis revealed a cluster of genes that were ... -
Nuclear monothiol glutaredoxins of Saccharomyces cerevisiae can function as mitochondrial glutaredoxins
Molina Navarro, Maria Micaela; Bellí i Martínez, Gemma; Torre Ruiz, M. A. de la; Rodríguez Manzanaque, Maria Teresa; Herrero Perpiñán, Enrique (The American Society for Biochemistry and Molecular Biology, 2004)Glutaredoxins are thiol oxidoreductases that regulate protein redox state. In Saccharomyces cerevisiae, Grx1 and Grx2 are cytosolic dithiol glutaredoxins, whereas Grx3, Grx4, and Grx5 are monothiol glutaredoxins. ... -
Physical interaction between the MAPK Slt2 of the PKC1-MAPK pathway and Grx3/Grx4 glutaredoxins is required for the oxidative stress response in budding yeast
Pujol Carrión, Núria; Torre Ruiz, M. A. de la (Elsevier, 2017)This study demonstrates that both monothiol glutaredoxins Grx3 and Grx4 physically interact with the MAPK Slt2 forming a complex involved in the cellular response to oxidative stress. The simultaneous absence of Grx3 and ... -
Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stress
Vilella Mitjana, Felipe; Herrero Perpiñán, Enrique; Torres Rosell, Jordi; Torre Ruiz, M. A. de la (The American Society for Biochemistry and Molecular Biology, 2005)In this study we analyze the participation of the PKC1-MAPK cell integrity pathway in cellular re- sponses to oxidative stress in Saccharomyces cerevisiae. Evidence is presented demonstrating that only Pkc1 and the ... -
Regulation of the cell integrity pathway by rapamycin-sensitive TOR function in budding yeast
Torres Rosell, Jordi; Di Como, Charles J.; Herrero Perpiñán, Enrique; Torre Ruiz, M. A. de la (The American Society for Biochemistry and Molecular Biology, 2002)The TOR (target of rapamycin) pathway controls cell growth in response to nutrient availability in eukary- otic cells. Inactivation of TOR function by rapamycin or nutrient exhaustion is accompanied by triggering various ... -
Signal flow between CWI/TOR and CWI/RAS in budding yeast under conditions of oxidative stress and glucose starvation
Petkova, Mima Ivanova; Pujol Carrión, Núria; Torre Ruiz, M. A. de la (Taylor & Francis; Landes Bioscience, 2010) -
Sit4 is required for proper modulation of the biological functions mediated by Pkc1 and the cell integrity pathway in Saccharomyces cerevisiae
Torre Ruiz, M. A. de la; Torres Rosell, Jordi; Ariño Carmona, Joaquín; Herrero Perpiñán, Enrique (The American Society for Biochemistry and Molecular Biology, 2002)Maintenance of cellular integrity in Saccharomyces cerevisiae is carried out by the activation of the protein kinase C-mediated mitogen-activated protein kinase (PKC1-MAPK) pathway. Here we report that correct down-regulation ... -
Structure-function analysis of yeast Grx5 monothiol glutaredoxin defines essential amino acids for the function of the protein
Bellí i Martínez, Gemma; Polaina, Julio; Tamarit Sumalla, Jordi; Torre Ruiz, M. A. de la; Rodríguez Manzanaque, Maria Teresa; Ros Salvador, Joaquim; Herrero Perpiñán, Enrique (The American Society for Biochemistry and Molecular Biology, 2002)Grx5 defines a family of yeast monothiol glutaredox- ins that also includes Grx3 and Grx4. All three proteins display significant sequence homology with proteins found from bacteria to humans. Grx5 is involved in ... -
The Cell Wall Integrity Receptor Mtl1 Contributes to Articulate Autophagic Responses When Glucose Availability Is Compromised
Montellá-Manuel, Sandra; Pujol Carrión, Núria; Torre Ruiz, M. A. de la (MDPI, 2021)Mtl1protein is a cell wall receptor belonging to the CWI pathway. Mtl1 function is related to glucose and oxidative stress signaling. In this report, we show data demonstrating that Mtl1 plays a critical role in the detection ... -
The MAP Kinase Slt2 Is Involved in Vacuolar Function and Actin Remodeling in Saccharomyces cerevisiae Mutants Affected by Endogenous Oxidative Stress
Pujol Carrión, Núria; Petkova, Mima Ivanova; Serrano Endolz, Luis; Torre Ruiz, M. A. de la (American Society for Microbiology, 2013)Oxidative stress causes transient actin cytoskeleton depolarization and also provokes vacuole fragmentation in wild-type cells. Under conditions of oxidative stress induced by hydrogen peroxide, the Slt2 protein is required ... -
Tor1, Sch9 and PKA downregulation in quiescence rely on Mtl1 to preserve mitochondrial integrity and cell survival
Sundaram, Venkatraghavan; Ivanova Petkova, Mima; Pujol Carrión, Núria; Boada Pallàs, Jordi; Torre Ruiz, M. A. de la (John Wiley & Sons, 2015-07-22)Here we show that Mtl1, member of the cell wall integrity pathway of Saccharomyces cerevisiae, plays a positive role in chronological life span (CLS). The absence of Mtl1 shortens CLS and causes impairment in the mitochondrial ...