Now showing items 1-20 of 26

    • 2-phenylethynesulphonamide (PFT-μ) enhances the anticancer effect of the novel hsp90 inhibitor NVP-AUY922 in melanoma, by reducing GSH levels 

      Yeramian Hakim, Andree; Veà Jódar, Àlvar; Benítez, Sandra; Ribera i Calvet, Joan; Domingo, Mónica; Santacana Espasa, Maria; Martínez Alonso, Montserrat; Maiques Carlos, Oscar; Valls Marsal, Joan; Dolcet Roca, Xavier; Vilella, Ramón; Cabiscol Català, Elisa; Matias-Guiu, Xavier; Marti, Rosa M. (Wiley online library, 2016)
      Heat shock proteins (HSPs), are molecular chaperones that assist the proper folding of nascent proteins. This study aims to evaluate the antitumour effects of the hsp90 inhibitor NVP-AUY922 in melanoma, both in vitro ...
    • Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington’s disease 

      Gomez Pastor, Rocio; Burchfiel, Eileen T.; Neef, Daniel W.; Jaeger, Alex M.; Cabiscol Català, Elisa; McKinstry, Spencer U.; Doss, Argenia; Aballay, Alejandro; Lo, Donald C.; Akimov, Sergey S.; Ross, Christopher A.; Eroglu, Cagla; Thiele, Dennis J. (Nature, 2017)
      Huntington’s Disease (HD) is a neurodegenerative disease caused by poly-glutamine expansion in the Htt protein, resulting in Htt misfolding and cell death. Expression of the cellular protein folding and pro-survival machinery ...
    • Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides 

      Tamarit Sumalla, Jordi; Hoogh, Anouk de; Obis Monné, Èlia; Alsina Obiols, David; Cabiscol Català, Elisa; Ros Salvador, Joaquim (Elsevier, 2012)
      Protein carbonyl detection has been commonly used to analyze the degree of damage to proteins under oxidative stress conditions. Most laboratories rely on derivatization of carbonyl groups with dinitrophenylhydrazine ...
    • Biochemical characterization of yeast mitochondrial Grx5 monothiol glutaredoxin 

      Tamarit Sumalla, Jordi; Bellí i Martínez, Gemma; Cabiscol Català, Elisa; Herrero Perpiñán, Enrique; Ros Salvador, Joaquim (The American Society for Biochemistry and Molecular Biology, 2003)
      Grx5 is a yeast mitochondrial protein involved in iron- sulfur biogenesis that belongs to a recently described family of monothiolic glutaredoxin-like proteins. No member of this family has been biochemically charac- terized ...
    • Chronological and replicative life-span extension in Saccharomyces cerevisiae by increased dosage of alcohol dehydrogenase 1 

      Reverter Branchat, Gemma; Cabiscol Català, Elisa; Tamarit Sumalla, Jordi; Sorolla Bardají, Maria Alba; Torre Ruiz, M. A. de la; Ros Salvador, Joaquim (Microbiology Society, 2007)
      Alcohol dehydrogenase 1 (Adh1)p catalyses the conversion of acetaldehyde to ethanol, regenerating NAD+. In Saccharomyces cerevisiae, Adh1p is oxidatively modified during ageing and, consequently, its activity becomes ...
    • Engineered Trx2p industrial yeast strain protects glycolysis and fermentation proteins from oxidative carbonylation during biomass propagation 

      Gómez-Pastor, Rocío; Pérez-Torrado, Roberto; Cabiscol Català, Elisa; Ros Salvador, Joaquim; Matallana, Emilia (BioMed Central, 2012)
      Background: In the yeast biomass production process, protein carbonylation has severe adverse effects since it diminishes biomass yield and profitability of industrial production plants. However, this significant detriment ...
    • Frataxin depletion in yeast triggers up-regulation of iron transport systems before affecting iron-sulfur enzyme activities 

      Moreno Cermeño, Armando J.; Obis Monné, Èlia; Bellí i Martínez, Gemma; Cabiscol Català, Elisa; Ros Salvador, Joaquim; Tamarit Sumalla, Jordi (The American Society for Biochemistry and Molecular Biology, 2010)
      The primary function of frataxin, a mitochondrial protein involved in iron homeostasis, remains controversial. Using a yeast model of conditional expression of the frataxin homologue YFH1, we analyzed the primary effects ...
    • Impaired PLP-dependent metabolism in brain samples from Huntington disease patients and transgenic R6/1 mice 

      Sorolla Bardají, Maria Alba; Rodríguez Colman, Maria José; Vall-llaura Espinosa, Núria; Vived Maza, Celia; Fernández Nogales, Marta; Lucas, José J.; Ferrer, Isidre; Cabiscol Català, Elisa (Springer Science, Business Media, 2016-06)
      Oxidative stress has been described as important to Huntington disease (HD) progression. In a previous HD study, we identified several carbonylated proteins, including pyridoxal kinase and antiquitin, both of which are ...
    • Major targets of iron-induced protein oxidative damage in frataxin-deficient yeasts are magnesium-binding proteins 

      Irazusta, Verónica Patricia; Moreno Cermeño, Armando J.; Cabiscol Català, Elisa; Ros Salvador, Joaquim; Tamarit Sumalla, Jordi (Elsevier, 2008)
      Iron accumulation has been associated with several pathological conditions such as Friedreich ataxia. This human disorder is caused by decreased expression of frataxin. Iron-overload triggers oxidative stress, but the ...
    • Metabolic remodeling in frataxin-deficient yeast is mediated by Cth2 and Adr1 

      Moreno Cermeño, Armando J.; Alsina Obiols, David; Cabiscol Català, Elisa; Tamarit Sumalla, Jordi; Ros Salvador, Joaquim (Elsevier, 2013)
      Frataxin is a mitochondrial protein involved in iron metabolism whose deficiency in humans causes Friedreich ataxia. We performed transcriptomic and proteomic analyses of conditional Yeast Frataxin Homologue (Yfh1) mutants ...
    • Mitochondrial Hsp60, resistance to oxidative stress, and the labile iron pool are closely connected in Saccharomyces cerevisiae 

      Cabiscol Català, Elisa; Bellí i Martínez, Gemma; Tamarit Sumalla, Jordi; Echave Lozano, Pedro; Herrero Perpiñán, Enrique; Ros Salvador, Joaquim (The American Society for Biochemistry and Molecular Biology, 2002)
      In the present study, we have analyzed the role of the molecular chaperone Hsp60 in protection of Saccharo- myces cerevisiae against oxidative damage. We con- structed mutant strains in which the levels of Hsp60 protein, ...
    • Mitochondrial localization of the yeast forkhead factor Hcm1 

      Rodríguez Colman, Maria José; Ros Salvador, Joaquim; Cabiscol Català, Elisa (MDPI, 2020-12)
      Hcm1 is a member of the forkhead transcription factor family involved in segregation, spindle pole dynamics, and budding in Saccharomyces cerevisiae. Our group described the role of Hcm1 in mitochondrial biogenesis and ...
    • Oxidative stress promotes specific protein damage in Saccharomyces cerevisiae 

      Cabiscol Català, Elisa; Piulats Combalia, Eva; Echave Lozano, Pedro; Herrero Perpiñán, Enrique; Ros Salvador, Joaquim (The American Society for Biochemistry and Molecular Biology, 2000)
      We have analyzed the proteins that are oxidatively damaged when Saccharomyces cerevisiae cells are ex- posed to stressing conditions. Carbonyl groups generated by hydrogen peroxide or menadione on proteins of aero- bically ...
    • Protein carbonylation: proteomics, specificity and relevance to aging 

      Cabiscol Català, Elisa; Tamarit Sumalla, Jordi; Ros Salvador, Joaquim (Wiley, 2013)
      Detection and quantification of protein carbonyls present in biological samples has become a popular, albeit indirect, method to determine the existence of oxidative stress. Moreover, the rise of proteomics has allowed ...
    • Protein oxidation in Huntington disease 

      Sorolla Bardají, Maria Alba; Rodríguez Colman, Maria José; Vall-llaura Espinosa, Núria; Tamarit Sumalla, Jordi; Ros Salvador, Joaquim; Cabiscol Català, Elisa (Wiley, 2012-05)
      Huntington disease (HD) is an inherited neurodegenerative disorder caused by expansion of CAG repeats in the huntingtin gene, affecting initially the striatum and progressively the cortex. Oxidative stress, and consequent ...
    • Protein oxidation in Huntington disease affects energy production and vitamin B6 metabolism 

      Sorolla Bardají, Maria Alba; Rodríguez Colman, Maria José; Tamarit Sumalla, Jordi; Ortega, Zaira; Lucas, José J.; Ferrer, Isidre; Ros Salvador, Joaquim; Cabiscol Català, Elisa (Elsevier, 2010)
      Huntington disease (HD) is an inherited neurodegenerative disorder that initially affects the striatum and progressively the cortex. Oxidative stress in HD has been described as important to disease progression. In ...
    • Proteomic and oxidative stress analysis in human brain samples of Huntington disease 

      Sorolla Bardají, Maria Alba; Reverter Branchat, Gemma; Tamarit Sumalla, Jordi; Ferrer, Isidre; Ros Salvador, Joaquim; Cabiscol Català, Elisa (Elsevier, 2008)
      Huntington disease (HD) is a neurodegenerative disorder caused by expansion of CAG repeats in exon 1 of the huntingtin gene, affecting initially the striatum and progressively the cortex. This work reports a proteomic analysis ...
    • Redox control and oxidative stress in yeast cells 

      Herrero Perpiñán, Enrique; Ros Salvador, Joaquim; Bellí i Martínez, Gemma; Cabiscol Català, Elisa (Elsevier, 2008)
      Protein structure and function can be altered by reactive oxygen species produced either by cell metabolism or by external oxidants. Although catalases, superoxide dismutases and peroxidases contribute to maintaining ...
    • Redox control of yeast Sir2 activity is involved in acetic acid resistance and longevity 

      Vall-llaura Espinosa, Núria; Mir, Noèlia; Garrido, Lourdes; Vived Maza, Celia; Cabiscol Català, Elisa (Elsevier, 2019-06)
      Yeast Sir2 is an NAD-dependent histone deacetylase related to oxidative stress and aging. In a previous study, we showed that Sir2 is regulated by S-glutathionylation of key cysteine residues located at the catalytic domain. ...
    • Reduction of oxidative cellular damage by overexpression of the thioredoxin TRX2 gene improves yield and quality of wine yeast dry active biomass 

      Gómez-Pastor, Rocío; Pérez-Torrado, Roberto; Cabiscol Català, Elisa; Ros Salvador, Joaquim; Matallana, Emilia (BioMed Central, 2010)
      Background: Wine Saccharomyces cerevisiae strains, adapted to anaerobic must fermentations, suffer oxidative stress when they are grown under aerobic conditions for biomass propagation in the industrial process of active ...