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dc.contributor.authorIzquierdo Álvarez, Alicia
dc.contributor.authorCasas Herranz, Celia
dc.contributor.authorHerrero Perpiñán, Enrique
dc.date.accessioned2014-07-25T10:52:59Z
dc.date.available2014-07-25T10:52:59Z
dc.date.issued2010
dc.identifier.issn2608–2620 (versió paper)
dc.identifier.issn1465-2080
dc.identifier.urihttp://hdl.handle.net/10459.1/47444
dc.description.abstractUnlike in higher organisms, selenium is not essential for growth in Saccharomyces cerevisiae. In this species, it causes toxic effects at high concentrations. In the present study, we show that when supplied as selenite to yeast cultures growing under fermentative metabolism, its effects can be dissected into two death phases. From the time of initial treatment, it causes loss of membrane integrity and genotoxicity. Both effects occur at higher levels in mutants lacking Grx1p and Grx2p than in wild-type cells, and are reversed by expression of a cytosolic version of the membrane-associated Grx7p glutaredoxin. Grx7p can also rescue the high levels of protein carbonylation damage that occur in selenite-treated cultures of the grx1 grx2 mutant. After longer incubation times, selenite causes abnormal nuclear morphology and the appearance of TUNEL-positive cells, which are considered apoptotic markers in yeast cells. This effect is independent of Grx1p and Grx2p. Therefore, the protective role of the two glutaredoxins is restricted to the initial stages of selenite treatment. Lack of Yca1p metacaspase or of a functional mitochondrial electron transport chain only moderately diminishes apoptotic-like death by selenite. In contrast, selenite-induced apoptosis is dependent on the apoptosis-inducing factor Aif1p. In the absence of the latter, intracellular protein carbonylation is reduced after prolonged selenite treatment, supporting the supposition that part of the oxidative damage is contributed by apoptotic cells.ca_ES
dc.language.isoengca_ES
dc.publisherSociety for General Microbiologyca_ES
dc.relation.isformatofVersió preprint del document publicat a: https://doi.org/10.1099/mic.0.039719-0ca_ES
dc.relation.ispartofMicrobiology, 2010, vol. 156, núm. 9, p. 2608-2620ca_ES
dc.rights(c) Society for General Microbiologyca_ES
dc.subjectLlevat de cervesaca_ES
dc.subjectGlutaredoxinca_ES
dc.subjectEstrès oxidatiuca_ES
dc.subject.otherSaccharomyces cerevisiaeca_ES
dc.subject.otherEstrès oxidatiuca_ES
dc.titleSelenite-induced cell death in Saccharomyces cerevisiae: protective role of glutaredoxinsca_ES
dc.typearticleca_ES
dc.identifier.idgrec16085
dc.type.versionsubmittedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1099/mic.0.039719-0


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