Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Producció Vegetal i Ciència Forestal
  • Articles publicats (Producció Vegetal i Ciència Forestal)
  • View Item
  •   Home
  • Recerca
  • Producció Vegetal i Ciència Forestal
  • Articles publicats (Producció Vegetal i Ciència Forestal)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Toxicity of allyl esters in insect cell lines and in Spodoptera littoralis larvae

Thumbnail
View/Open
017822.pdf (548.5Kb)
Sol·licita una còpia
Issue date
2012
Author
Giner Gil, Marta
Avilla Hernández, Jesús
Balcells Fluvià, Mercè
Caccia, Silvia
Smagghe, Guy
Suggested citation
Giner Gil, Marta; Avilla Hernández, Jesús; Balcells Fluvià, Mercè; Caccia, Silvia; Smagghe, Guy; . (2012) . Toxicity of allyl esters in insect cell lines and in Spodoptera littoralis larvae. Archives of Insect Biochemistry and Physiology, 2012, vol. 79, num. 1, p. 18-30. https://doi.org/10.1002/arch.21002.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
We investigated the effects of five allyl esters, two aromatic (allyl cinnamate and allyl 2-furoate) and three aliphatic (allyl hexanoate, allyl heptanoate, and allyl octanoate) in established insect cell lines derived from different species and tissues. We studied embryonic cells of the fruit fly Drosophila melanogaster (S2) (Diptera) and the beet armyworm Spodoptera exigua (Se4) (Lepidoptera), fat body cells of the Colorado potato beetle Leptinotarsa decemlineata (CPB) (Coleoptera), ovarian cells of the silkmoth Bombyx mori (Bm5), and midgut cells of the spruce budworm Choristoneura fumiferana (CF203) (Lepidoptera). Cytotoxicity was determined with use of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and trypan blue. In addition, we tested the entomotoxic action of allyl cinnamate against the cotton leafworm Spodoptera littoralis .The median (50%) cytotoxic concentrations (EC50s) of the five allyl esters in the MTT bioassays ranged between 0.25 and 27 mM with significant differences among allyl esters (P = 0.0012), cell lines (P < 0.0001), and the allyl ester<br>cell line interaction (P < 0.0001). Allyl cinnamate was the most active product, and CF203 the most sensitive cell line. In the trypan blue bioassays, cytotoxicity was produced rapidly and followed the same trend observed in the MTT bioassay. In first instars of S. littoralis, allyl cinnamate killed all larvae at 0.25% in the diet after 1 day, while this happened in third instars after 5 days. The LC50 in first instars was 0.08%. In addition, larval weight gain was reduced (P < 0.05) after 1 day of feeding on diet with 0.05%. In conclusion, the data provide evidence of the significant but differential cytotoxicity among allyl esters in insect cells of different species and tissues. Midgut cells show high sensitivity, indicating the insect midgut as a primary target tissue. Allyl cinnamate caused rapid toxic effects in S. littoralis larvae at low concentrations, suggesting further potential for use in pest control.
URI
http://hdl.handle.net/10459.1/47371
DOI
https://doi.org/10.1002/arch.21002
Is part of
Archives of Insect Biochemistry and Physiology, 2012, vol. 79, num. 1, p. 18-30
European research projects
Collections
  • Articles publicats (Producció Vegetal i Ciència Forestal) [772]

Contact Us | Send Feedback | Legal Notice
© 2022 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2022 BiD. Universitat de Lleida
Metadata subjected to