Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy
Reproductive traits play a key role in pig production in order to reduce costs and increase economic returns. Among others, gene expression analyses represent a useful approach to study genetic mechanisms underlying reproductive traits in pigs. The application of reverse-transcription quantitative PCR requires the selection of appropriate reference genes, whose expression levels should not be affected by the experimental conditions, especially when comparing gene expression across different physiological stages.
Journal or Serie
PLoS ONE, 2014, vol.8, núm.6, e66023