A functional variant in the Stearoyl-CoA desaturase gene promoter enhances fatty acid desaturation in pork

View/ Open
Issue date
2014Suggested citation
Estany Illa, Joan;
Ros Freixedes, Roger;
Tor i Naudí, Marc;
Pena i Subirà, Ramona Natacha;
.
(2014)
.
A functional variant in the Stearoyl-CoA desaturase gene promoter enhances fatty acid desaturation in pork.
PLoS ONE, 2014, vol.9, núm.1, e86177.
https://doi.org/10.1371/journal.pone.0086177.
Metadata
Show full item recordAbstract
There is growing public concern about reducing saturated fat intake. Stearoyl-CoA desaturase (SCD) is the lipogenic enzyme responsible for the biosynthesis of oleic acid (18:1) by desaturating stearic acid (18:0). Here we describe a total of 18 mutations in the promoter and 3′ non-coding region of the pig SCD gene and provide evidence that allele T at AY487830:g.2228T>C in the promoter region enhances fat desaturation (the ratio 18:1/18:0 in muscle increases from 3.78 to 4.43 in opposite homozygotes) without affecting fat content (18:0+18:1, intramuscular fat content, and backfat thickness). No mutations that could affect the functionality of the protein were found in the coding region. First, we proved in a purebred Duroc line that the C-T-A haplotype of the 3 single nucleotide polymorphisms (SNPs) (g.2108C>T; g.2228T>C; g.2281A>G) of the promoter region was additively associated to enhanced 18:1/18:0 both in muscle and subcutaneous fat, but not in liver. We show that this association was consistent over a 10-year period of overlapping generations and, in line with these results, that the C-T-A haplotype displayed greater SCD mRNA expression in muscle. The effect of this haplotype was validated both internally, by comparing opposite homozygote siblings, and externally, by using experimental Duroc-based crossbreds. Second, the g.2281A>G and the g.2108C>T SNPs were excluded as causative mutations using new and previously published data, restricting the causality to g.2228T>C SNP, the last source of genetic variation within the haplotype. This mutation is positioned in the core sequence of several putative transcription factor binding sites, so that there are several plausible mechanisms by which allele T enhances 18:1/18:0 and, consequently, the proportion of monounsaturated to saturated fat.
Is part of
PLoS ONE, 2014, vol.9, núm.1, e86177European research projects
Collections
The following license files are associated with this item:
Related items
Showing items related by title, author, creator and subject.
-
Leptin receptor and fatty acid desaturase-2 gene variants affect fat, color and production profile of dry-cured hams
Suárez-Mesa, Rafael; Ros Freixedes, Roger; Tor i Naudí, Marc; Reixach, Josep; Pena i Subirà, Ramona Natacha; Estany Illa, Joan (Elsevier, 2020-12-03)The effects of LEPR (rs709596309C>T) and FADS2 (rs321384923A>G) single nucleotide polymorphisms on production and quality attributes in purebred Duroc dry-cured hams were examined. As compared to LEPR-C- hams, the LEPR-TT ... -
A polymorphism in the stearoyl-CoA desaturase gene promoter increases monounsaturated fatty acid content in dry-cured ham
Henriquez Rodriguez, Eliana; Tor i Naudí, Marc; Pena i Subirà, Ramona Natacha; Estany Illa, Joan (Elsevier, 2015)Data on 125 dry-cured hams from purebred Duroc pigs were used to examine whether the favorable effect of the T allele in the promoter region of the stearoyl-CoA desaturase gene (AY487830:g.2228T>C) on monounsaturated fatty ... -
A polymorphism in the stearoyl-CoA desaturase gene promoter influences monounsaturated fatty acid content of Duroc × Iberian hams
Henriquez Rodriguez, Eliana; Tor i Naudí, Marc; Pena i Subirà, Ramona Natacha; Estany Illa, Joan (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2015)Data on 74 dry-cured hams from Duroc × Iberian pigs were used to examine whether the tag polymorphism AY487830:g.2228T>C in the promoter region of the stearoyl-CoA desaturase [SCD] gene affect fat desaturation and ...