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dc.contributor.authorPujol Carrión, Núria
dc.contributor.authorBellí i Martínez, Gemma
dc.contributor.authorHerrero Perpiñán, Enrique
dc.contributor.authorNogues, Antoni
dc.contributor.authorTorre Ruiz, M. A. de la
dc.date.accessioned2014-05-19T14:46:43Z
dc.date.available2014-05-19T14:46:43Z
dc.date.issued2006
dc.identifier.issn0021-9533 (versió paper)
dc.identifier.issn1477-9137
dc.identifier.urihttp://hdl.handle.net/10459.1/47222
dc.description.abstractGrx3 and Grx4, two monothiol glutaredoxins of Saccharomyces cerevisiae, regulate Aft1 nuclear localisation. We provide evidence of a negative regulation of Aft1 activity by Grx3 and Grx4. The Grx domain of both proteins played an important role in Aft1 translocation to the cytoplasm. This function was not, however, dependent on the availability of iron. Here we demonstrate that Grx3, Grx4 and Aft1 interact each other both in vivo and in vitro, which suggests the existence of a functional protein complex. Interestingly, each interaction occurred independently on the third member of the complex. The absence of both Grx3 and Grx4 induced a clear enrichment of G1 cells in asynchronous cultures, a slow growth phenotype, the accumulation of intracellular iron and a constitutive activation of the genes regulated by Aft1. The grx3grx4 double mutant was highly sensitive to the oxidising agents hydrogen peroxide and t-butylhydroperoxide but not to diamide. The phenotypes of the double mutant grx3grx4 characterised in this study were mainly mediated by the Aft1 function, suggesting that grx3grx4 could be a suitable cellular model for studying endogenous oxidative stress induced by deregulation of the iron homeostasis. However, our results also suggest that Grx3 and Grx4 might play additional roles in the oxidative stress response through proteins other than Aft1.ca_ES
dc.language.isoengca_ES
dc.publisherCompany of Biologistsca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1242/jcs.03229ca_ES
dc.relation.ispartofJournal of cell science, 2006, vol. 119, núm. 21, p. 4554-4564ca_ES
dc.rights(c) Company of Biologistsca_ES
dc.subjectGrx3ca_ES
dc.subjectGrx4ca_ES
dc.subjectAft1ca_ES
dc.subject.otherBiologia molecularca_ES
dc.subject.otherEstrès oxidatiuca_ES
dc.subject.otherCicle cel·lularca_ES
dc.subject.otherBioquímicaca_ES
dc.titleGlutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiaeca_ES
dc.typearticleca_ES
dc.identifier.idgrec11148
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1242/jcs.03229


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