Grx5 glutaredoxin plays a central role in protection against protein oxidative damage in Saccharomyces cerevisiae
Rodríguez Manzanaque, Maria Teresa
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Glutaredoxins are members of a superfamily of thiol disulfide oxidoreductases involved in maintaining the redox state of target proteins. In Saccharomyces cerevisiae, two glutaredoxins (Grx1 and Grx2) containing a cysteine pair at the active site had been characterized as protecting yeast cells against oxidative damage. In this work, another subfamily of yeast glutaredoxins (Grx3, Grx4, and Grx5) that differs from the first in containing a single cysteine residue at the putative active site is described. This trait is also characteristic for a number of glutaredoxins from bacteria to humans, with which the Grx3/4/5 group has extensive homology over two regions. Mutants lacking Grx5 are partially deficient in growth in rich and minimal media and also highly sensitive to oxidative damage caused by menadione and hydrogen peroxide. A significant increase in total protein carbonyl content is constitutively observed in grx5cells, and a number of specific proteins, including transketolase, appear to be highly oxidized in this mutant. The synthetic lethality of the grx5 and grx2 mutations on one hand and ofgrx5 with the grx3 grx4 combination on the other points to a complex functional relationship among yeast glutaredoxins, with Grx5 playing a specially important role in protection against oxidative stress both during ordinary growth conditions and after externally induced damage. Grx5-deficient mutants are also sensitive to osmotic stress, which indicates a relationship between the two types of stress in yeast cells.
Is part ofMolecular and Cellular Biology, 1999, vol. 19, núm. 12, p. 8180-8190
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Grx5 glutaredoxin plays a central role in protection against protein oxidative damage in Saccharomyces cerevisiae Rodríguez-Manzaneque Martínez, María Teresa; Ros i Salvador, Joaquim; Cabiscol Català, Elisa; Sorribas Tello, Albert; Herrero Perpiñán, Enrique (1999)Glutaredoxins are members of a superfamily of thiol disulfide oxidoreductases involved in maintaining the redox state of target proteins. In Saccharomyces cerevisiae, two glutaredoxins (Grx1 and Grx2) containing a cysteine ...
Cabiscol Català, Elisa; Piulats Combalia, Eva; Echave Lozano, Pedro; Herrero Perpiñán, Enrique; Ros Salvador, Joaquim (The American Society for Biochemistry and Molecular Biology, 2000)We have analyzed the proteins that are oxidatively damaged when Saccharomyces cerevisiae cells are ex- posed to stressing conditions. Carbonyl groups generated by hydrogen peroxide or menadione on proteins of aero- bically ...
Structure-function analysis of yeast Grx5 monothiol glutaredoxin defines essential amino acids for the function of the protein Bellí i Martínez, Gemma; Polaina, Julio; Tamarit Sumalla, Jordi; Torre Ruiz, M. A. de la; Rodríguez Manzanaque, Maria Teresa; Ros Salvador, Joaquim; Herrero Perpiñán, Enrique (The American Society for Biochemistry and Molecular Biology, 2002)Grx5 defines a family of yeast monothiol glutaredox- ins that also includes Grx3 and Grx4. All three proteins display significant sequence homology with proteins found from bacteria to humans. Grx5 is involved in ...