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dc.contributor.authorRivera Vélez, Sol Maiam
dc.contributor.authorCanela i Garayoa, Ramon
dc.date.accessioned2014-01-23T18:23:54Z
dc.date.available2014-01-23T18:23:54Z
dc.date.issued2012
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10459.1/46963
dc.description.abstractWe performed a number of tests with the aim to develop an effective extraction method for the analysis of carotenoid content in maize seed. Mixtures of methanol–ethyl acetate (6:4, v/v) and methanol–tetrahydrofuran (1:1, v/v) were the most effective solvent systems for carotenoid extraction from maize endosperm under the conditions assayed. In addition, we also addressed sample preparation prior to the analysis of carotenoids by liquid chromatography (LC). The LC response of extracted carotenoids and standards in several solvents was evaluated and results were related to the degree of solubility of these pigments. Three key factors were found to be important when selecting a suitable injection solvent: compatibility between the mobile phase and injection solvent, carotenoid polarity and content in the matrix.ca_ES
dc.description.sponsorshipThis work was supported by the University of Lleida, Ministry of Science and Innovation (MICINN), Spain (BFU200761413 and CTQ2009-14699-C02-01), and an ERC Advanced Grant (BIOFORCE) to Paul Christou.
dc.language.isoengca_ES
dc.publisherMolecular Diversity Preservation Internationalca_ES
dc.relationMIECI/PN2004-2007/BFU200761413
dc.relationMICINN/PN2008-2011/CTQ2009-14699-C02-01
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.3390/molecules170911255ca_ES
dc.relation.ispartofMolecules, 2012, vol. 17, núm. 9, p. 11255-11268ca_ES
dc.rightscc-by, (c) Rivera et al., 2012ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/deed.ca
dc.subjectCarotenoidsca_ES
dc.subjectExtractionca_ES
dc.subjectSolubilityca_ES
dc.subjectInjection solventca_ES
dc.subjectLiquid chromatographyca_ES
dc.subject.otherBlat de moro -- Anàlisica_ES
dc.titleInfluence of sample processing on the analysis of carotenoids in maizeca_ES
dc.typearticleca_ES
dc.identifier.idgrec018214
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.3390/molecules170911255


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cc-by, (c) Rivera et al., 2012
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