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dc.contributor.authorGarcés, Josep Lluís
dc.contributor.authorMas i Pujadas, Francesc
dc.contributor.authorPuy Llorens, Jaume
dc.date.accessioned2013-03-04T09:31:58Z
dc.date.available2013-03-04T09:31:58Z
dc.date.issued1999
dc.identifier.issn0021-9606
dc.identifier.urihttp://hdl.handle.net/10459.1/46475
dc.description.abstractThis paper presents an approach based on the saddle-point approximation to study the equilibrium interactions between small molecules and macromolecules with a large number of sites. For this case, the application of the Darwin–Fowler method results in very simple expressions for the stoichiometric equilibrium constants and their corresponding free energies in terms of integrals of the binding curve plus a correction term which depends on the first derivatives of the binding curve in the points corresponding to an integer value of the mean occupation number. These expressions are simplified when the number of sites tends to infinity, providing an interpretation of the binding curve in terms of the stoichiometric stability constants. The formalism presented is applied to some simple complexation models, obtaining good values for the free energies involved. When heterogeneous complexation is assumed, simple expressions are obtained to relate the macroscopic description of the binding, given by the stoichiomeric constants, with the microscopic description in terms of the intrinsic stability constants or the affinity spectrum. © 1999 American Institute of Physics.ca_ES
dc.language.isoengca_ES
dc.publisherAmerican Institute of Physicsca_ES
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1063/1.479559ca_ES
dc.relation.ispartofJournal of Chemical Physics, 1999, vol. 111, núm. 6, p. 2818-2828ca_ES
dc.rights(c) American Institute of Physics, 1999ca_ES
dc.subjectMacromoleculesca_ES
dc.subjectFree energyca_ES
dc.subjectStoichiometryca_ES
dc.subjectMolecular biophysicsca_ES
dc.subject.otherMacromolèculesca_ES
dc.subject.otherEstequiometriaca_ES
dc.subject.otherBiologia molecularca_ES
dc.subject.otherBiofísicaca_ES
dc.titleComplexation to macromolecules with a large number of sitesca_ES
dc.typearticleca_ES
dc.identifier.idgrec000717
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1063/1.479559


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