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dc.contributor.authorRodríguez García, Marcela
dc.contributor.authorMarques, Tânia
dc.contributor.authorBosch Serra, Dolors
dc.contributor.authorAvilla Hernández, Jesús
dc.date.accessioned2013-02-21T10:26:05Z
dc.date.available2013-02-21T10:26:05Z
dc.date.issued2011
dc.identifier.issn0048-3575
dc.identifier.urihttp://hdl.handle.net/10459.1/46462
dc.description.abstractSpanish Cydia pomonella (L.) field populations have developed resistance to several insecticide groups. Diagnostic concentrations were established as the LC90 calculated on a susceptible strain (S_Spain) for five and seven insecticides and tested on eggs and neonate larvae field populations, respectively. The three most relevant enzymatic detoxification systems (mixed-function oxidases (MFO), glutathione S-tranferases (GST) and esterases (EST)) were studied for neonate larvae. In eggs, 96% of the field populations showed a significantly lower efficacy when compared with the susceptible strain (S_Spain) and the most effective insecticides were fenoxycarb and thiacloprid. In neonate larvae, a significant loss of susceptibility to the insecticides was detected. Flufenoxuron, azinphos-methyl and phosmet showed the lowest efficacy, while lambda-cyhalothrin, alpha-cypermethrin and chlorpyrifos-ethyl showed the highest. Biochemical assays showed that the most important enzymatic system involved in insecticide detoxification was MFO, with highest enzymatic activity ratios (5.1–16.6 for neonates from nine field populations). An enhanced GST and EST activities was detected in one field population, with enzymatic activity ratios of threefold and fivefold for GST and EST, respectively, when compared with the susceptible strain. The insecticide bioassays showed that the LC90 used were effective as diagnostic concentrations. Measures of MFO activity alongside bioassays with insecticide diagnostic concentrations could be used as tools for monitoring insecticide resistance in neonate larvae of C. pomonella.ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.pestbp.2011.03.003ca_ES
dc.relation.ispartofPesticide Biochemistry and Physiology, 2011, vol. 100, núm. 2, p. 151-159ca_ES
dc.rights(c) Elsevier, 2011ca_ES
dc.subjectEggsca_ES
dc.subjectNeonate larvaeca_ES
dc.subjectInsecticide resistanceca_ES
dc.subjectDetoxifying enzymesca_ES
dc.subject.otherCydia pomonellaca_ES
dc.subject.otherPomera -- Malalties i plaguesca_ES
dc.titleAssessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)ca_ES
dc.typearticleca_ES
dc.identifier.idgrec017164
dc.type.versionacceptedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.pestbp.2011.03.003


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