Neuronal survival induced by neurotrophins requires calmodulin

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2001
Author
Egea Navarro, Joaquim
Espinet Mestre, Carme
Soler i Tatché, Rosa Ma.
Dolcet Roca, Xavier
Yuste Mateos, Víctor J. (Víctor José)
Encinas Martín, Mario
Iglesias Durán, Montserrat
Rocamora Ibars, Nativitat
Comella i Carnicé, Joan Xavier
Suggested citation
Egea Navarro, Joaquim; Espinet Mestre, Carme; Soler i Tatché, Rosa Ma.; Dolcet Roca, Xavier; Yuste Mateos, Víctor J. (Víctor José); Encinas Martín, Mario; ... Comella i Carnicé, Joan Xavier. (2001) . Neuronal survival induced by neurotrophins requires calmodulin. Journal of Cell Biology, 2001, vol. 154, núm. 3, p. 585–597. https://doi.org/10.1083/jcb.200101023.
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Abstract
It has been reported that phosphoinositide 3-kinase (PI 3-kinase) and its downstream target, protein kinase B (PKB), play a central role in the signaling of cell survival triggered by neurotrophins (NTs). In this report, we have analyzed the involvement of Ca2+ and calmodulin (CaM) in the activation of the PKB induced by NTs. We have found that reduction of intracellular Ca2+ concentration or functional blockade of CaM abolished NGF-induced activation of PKB in PC12 cells. Similar results were obtained in cultures of chicken spinal cord motoneurons treated with brain-derived neurotrophic factor (BDNF). Moreover, CaM inhibition prevented the cell survival triggered by NGF or BDNF. This effect was counteracted by the transient expression of constitutive active forms of the PKB, indicating that CaM regulates NT-induced cell survival through the activation of the PKB. We have investigated the mechanisms whereby CaM regulates the activation of the PKB, and we have found that CaM was necessary for the proper generation and/or accumulation of the products of the PI 3-kinase in intact cells.
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http://hdl.handle.net/10459.1/46380
DOI
https://doi.org/10.1083/jcb.200101023
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Journal of Cell Biology, 2001, vol. 154, núm. 3, p. 585–597
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