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dc.contributor.authorCánovas Tienda, Angela
dc.contributor.authorPena i Subirà, Ramona Natacha
dc.contributor.authorGallardo García, David
dc.contributor.authorRamírez Bellido, Óscar
dc.contributor.authorAmills i Eras, Marcel
dc.contributor.authorQuintanilla Aguado, Raquel
dc.date.accessioned2012-12-13T12:27:03Z
dc.date.available2012-12-13T12:27:03Z
dc.date.issued2012
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10459.1/46374
dc.description.abstractBackground: The main goal of the present study was to analyse the genetic architecture of mRNA expression in muscle, a tissue with an outmost economic importance for pig breeders. Previous studies have used F2 crosses to detect porcine expression QTL (eQTL), so they contributed with data that mostly represents the between-breed component of eQTL variation. Herewith, we have analysed eQTL segregation in an outbred Duroc population using two groups of animals with divergent fatness profiles. This approach is particularly suitable to analyse the within-breed component of eQTL variation, with a special emphasis on loci involved in lipid metabolism. Methodology/Principal Findings: GeneChip Porcine Genome arrays (Affymetrix) were used to determine the mRNA expression levels of gluteus medius samples from 105 Duroc barrows. A whole-genome eQTL scan was carried out with a panel of 116 microsatellites. Results allowed us to detect 613 genome-wide significant eQTL unevenly distributed across the pig genome. A clear predominance of trans- over cis-eQTL, was observed. Moreover, 11 trans-regulatory hotspots affecting the expression levels of four to 16 genes were identified. A Gene Ontology study showed that regulatory polymorphisms affected the expression of muscle development and lipid metabolism genes. A number of positional concordances between eQTL and lipid trait QTL were also found, whereas limited evidence of a linear relationship between muscle fat deposition and mRNA levels of eQTL regulated genes was obtained. Conclusions/Significance: Our data provide substantial evidence that there is a remarkable amount of within-breed genetic variation affecting muscle mRNA expression. Most of this variation acts in trans and influences biological processes related with muscle development, lipid deposition and energy balance. The identification of the underlying causal mutations and the ascertainment of their effects on phenotypes would allow gaining a fundamental perspective about how complex traits are built at the molecular level.ca_ES
dc.language.isoengca_ES
dc.publisherPublic Library of Science (PLoS)
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0035583ca_ES
dc.relation.ispartofPLoS ONE, 2012, vol. 7, núm. 4, e35583ca_ES
dc.rightscc-by, (c) Cánovas et al., 2012ca_ES
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/es/deed.caca_ES
dc.subject.otherPorcs -- Genèticaca_ES
dc.subject.otherRNAca_ES
dc.titleSegregation of regulatory polymorphisms with effects on the gluteus medius transcriptome in a purebred pig populationca_ES
dc.typearticleca_ES
dc.identifier.idgrec017950
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0035583


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cc-by, (c) Cánovas et al., 2012
Except where otherwise noted, this item's license is described as cc-by, (c) Cánovas et al., 2012