The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages

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2010
Author
Bermúdez López, Marcelino
Ceschia, Audrey
Piccoli, Giacomo De
Colomina i Gabarrella, Neus
Pasero, Philippe
Aragón, Luis
Torres Rosell, Jordi
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Abstract
Mitotic chromosome segregation requires the removal of physical connections between sister chromatids. In addition to cohesin and topological entrapments, sister chromatid separation can be prevented by the presence of chromosome junctions or ongoing DNA replication. We will collectively refer to them
as DNA-mediated linkages. Although this type of structures has been documented in different DNA replication and repair mutants, there is no known essential mechanism ensuring their timely removal before mitosis. Here, we show that the dissolution of these connections is an active process that requires the Smc5/6 complex, together with Mms21, its associated SUMO-ligase. Failure to remove DNA-mediated linkages causes gross chromosome missegregation in anaphase. Moreover, we show that Smc5/6 is capable to dissolve them in metaphase-arrested cells, thus restoring chromosome resolution and segregation. We propose that Smc5/6 has an essential role in the removal of DNA-mediated linkages to prevent chromosome missegregation and aneuploidy.
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http://hdl.handle.net/10459.1/46368
DOI
https://doi.org/10.1093/nar/gkq546
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Nucleic Acids Research, 2010, vol. 38, núm. 19, p. 6502-6512
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cc-by-nc, (c) Bermúdez-López et al., 2010
Except where otherwise noted, this item's license is described as cc-by-nc, (c) Bermúdez-López et al., 2010