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dc.contributor.authorGarza, María Carmen
dc.contributor.authorFernández Borges, Natalia
dc.contributor.authorBolea, Rosa
dc.contributor.authorBadiola, Juan José
dc.contributor.authorCastilla, Joaquín
dc.contributor.authorMonleón Moscardó, Eva
dc.description.abstractScrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.ca_ES
dc.description.sponsorshipThis study was supported by a national grant from Spain (AGL2009-11553-C02-01), a Basque government grant (PI2010-18) and the Department of Industry, Tourism and Trade of the Government of the Autonomous Community of the Basque Country (Etortek Research Programs 2011/2013), from the Innovation Technology Department of the Bizkaia County. Dr. Garza was supported by a FPU doctoral grant from the Spanish Ministry of Education (AP2007-03842). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.publisherPublic Library of Science (PLoS)ca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofPLoS ONE, 2011, vol. 6, núm. 12, e27525ca_ES
dc.rightscc-by, (c) Garza et al., 2011ca_ES
dc.subject.otherEncefalopatia espongiforme bovinaca_ES
dc.subject.otherSistema nerviós central -- Malaltiesca_ES
dc.titleDetection of PrPres in genetically susceptible fetuses from sheep with natural scrapieca_ES

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