Show simple item record

dc.contributor.authorRodríguez-Manzaneque Martínez, María Teresa
dc.contributor.authorTamarit Sumalla, Jordi
dc.contributor.authorBellí i Martínez, Gemma
dc.contributor.authorRos Salvador, Joaquim
dc.contributor.authorHerrero Perpiñán, Enrique
dc.identifier.issn1059-1524 (versió paper)
dc.description.abstractYeast cells contain a family of three monothiol glutaredoxins: Grx3, 4, and 5. Absence of Grx5 leads to constitutive oxidative damage, exacerbating that caused by external oxidants. Phenotypic defects associated with the absence of Grx5 are suppressed by overexpression ofSSQ1 and ISA2, two genes involved in the synthesis and assembly of iron/sulfur clusters into proteins. Grx5 localizes at the mitochondrial matrix, like other proteins involved in the synthesis of these clusters, and the mature form lacks the first 29 amino acids of the translation product. Absence of Grx5 causes: 1) iron accumulation in the cell, which in turn could promote oxidative damage, and 2) inactivation of enzymes requiring iron/sulfur clusters for their activity. Reduction of iron levels in grx5 null mutants does not restore the activity of iron/sulfur enzymes, and cell growth defects are not suppressed in anaerobiosis or in the presence of disulfide reductants. Hence, Grx5 forms part of the mitochondrial machinery involved in the synthesis and assembly of iron/sulfur centers.ca_ES
dc.publisherAmerican Society for Cell Biologyca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofMolecular Biology of the Cell, 2002, vol. 13, núm. 4, p. 1109 –1121ca_ES
dc.rightscc-by-nc-sa, (c) Rodríguez-Manzaneque et al., 2002ca_ES
dc.subject.otherEstrès oxidatiuca_ES
dc.subject.otherProteïnes -- Anàlisica_ES
dc.subject.otherFerro -- Aspectes fisiològicsca_ES
dc.subject.otherLlevat de cervesaca_ES
dc.subject.otherSaccharomyces -- Citologiaca_ES
dc.titleGrx5 is a mitochondrial glutaredoxin required for the activity of iron/sulfur enzymesca_ES

Files in this item


This item appears in the following Collection(s)

Show simple item record

cc-by-nc-sa, (c) Rodríguez-Manzaneque et al., 2002
Except where otherwise noted, this item's license is described as cc-by-nc-sa, (c) Rodríguez-Manzaneque et al., 2002