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dc.contributor.authorCastells i Roca, Laia
dc.contributor.authorGarcía-Martínez, José
dc.contributor.authorMoreno, Joaquín
dc.contributor.authorHerrero Perpiñán, Enrique
dc.contributor.authorBellí i Martínez, Gemma
dc.contributor.authorPérez-Ortín, José E.
dc.description.abstractWe have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25uC to 37uC. Using an established mathematical algorithm, theoretical mRNA decay rates have also been calculated from the experimental data. We have verified the mathematical predictions for selected genes by determining their mRNA decay rates at different times during heat stress response using the regulatable tetO promoter. This study indicates that the yeast response to heat shock is not only due to changes in transcription rates, but also to changes in the mRNA stabilities. mRNA stability is affected in 62% of the yeast genes and it is particularly important in shaping the mRNA profile of the genes belonging to the environmental stress response. In most cases, changes in transcription rates and mRNA stabilities are homodirectional for both parameters, although some interesting cases of antagonist behavior are found. The statistical analysis of gene targets and sequence motifs within the clusters of genes with similar behaviors shows that both transcriptional and post-transcriptional regulons apparently contribute to the general heat stress response by means of transcriptional factors and RNA binding proteins.ca_ES
dc.publisherPublic Library of Science (PLoS)ca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofPLoS ONE, 2011, vol. 6, núm. 2, e17272ca_ES
dc.rightscc-by, (c) Castells-Roca et al., 2011ca_ES
dc.subject.otherLlevats -- Genèticaca_ES
dc.subject.otherLlevat de cervesaca_ES
dc.subject.otherEstrès oxidatiuca_ES
dc.subject.otherTranscripció genèticaca_ES
dc.titleHeat shock response in yeast involves changes in both transcription rates and mRNA stabilitiesca_ES

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cc-by, (c) Castells-Roca et al., 2011
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