Now showing items 1-20 of 51

    • A golden era—pro-vitamin A enhancement in diverse crops 

      Bai, Chao; Twyman, Richard M.; Farré Martinez, Gemma; Sanahuja Solsona, Georgina; Christou, Paul; Capell Capell, Teresa; Zhu, Changfu (Springer Verlag, 2011)
      Numerous crops have been bred or engineered to increase carotenoid levels in an effort to develop novel strategies that address vitamin A deficiency in the developing world. The pioneering work in rice (not covered in ...
    • A novel carotenoid, 4-keto-a-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callus 

      Breitenbach, Jürgen; Bai, Chao; Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu; Sandmann, Gerhard (Elsevier, 2014)
      Rice endosperm is devoid of carotenoids because the initial biosynthetic steps are absent. The early carotenogenesis reactions were constituted through co-transformation of endosperm-derived rice callus with phytoene ...
    • A question of balance: achieving appropriate nutrient levels in biofortified staple crops 

      Sanahuja Solsona, Georgina; Farré Martinez, Gemma; Berman Quintana, Judit; Zorrilla López, Uxue; Twyman, Richard M.; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu (Cambridge University Press, 2013)
      The biofortification of staple crops with vitamins is an attractive strategy to increase the nutritional quality of human food, particularly in areas where the population subsists on a cereal-based diet. Unlike other ...
    • An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation 

      Bai, Chao; Rivera Vélez, Sol Maiam; Medina Piles, Vicente; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Capell Capell, Teresa; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2014)
      We have developed an assay based on rice embryogenic callus for rapid functional characterization of metabolic genes. We validated the assay using a selection of well-characterized genes with known functions in the carotenoid ...
    • Ascorbic acid synthesis and metabolism in maize are subject to complex and genotype-dependent feedback regulation duringendosperm development 

      Sanahuja Solsona, Georgina; Farré Martinez, Gemma; Bassie Rene, Ludovic; Zhu, Changfu; Christou, Paul; Capell Capell, Teresa (Wiley, 2013)
      L-ascorbic acid (vitamin C) is an antioxidant and electron donor whose metabolism in plants isunder strict feedback control. The factors that influence L-ascorbic acid accumulation in staplecrops are only partially understood. ...
    • Background, current status and future prospects of transgenic crop plant development 

      Capell Capell, Teresa; Zhu, Changfu; Bassie Rene, Ludovic; Peremartí, Ariadna; Naqvi, Shaista; Ramessar, Koreen; Gómez Galera, Sonia; Christou, Paul (Institut d'Estudis Catalans. Secció de Ciències i Tecnologia, 2007)
      Les plantes transgèniques són una part integral de l’agricultura contemporània. Durant l’any 2006 més de noranta milions d’hectàrees de plantes transgèniques van ser cultivades en vint-i-un països. Des de la comercialització ...
    • Biofortification of crops with nutrients: factors affecting utilization and storage 

      Díaz Gómez, Joana; Twyman, Richard M.; Zhu, Changfu; Farré Martinez, Gemma; Serrano Casasola, José Carlos Enrique; Portero Otín, Manuel; Muñoz Odina, Mª Pilar; Sandmann, Gerhard; Capell Capell, Teresa; Christou, Paul (Elsevier, 2017-01-06)
      Biofortification is an effective and economical method to improve the micronutrient content of crops, particularly staples that sustain human populations in developing countries. Whereas conventional fortification requires ...
    • Bottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor–product balance 

      Bai, Chao; Capell Capell, Teresa; Berman Quintana, Judit; Medina Piles, Vicente; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2016)
      The profile of secondary metabolites in plants reflects the balance of biosynthesis, degradation and storage, including the availability of precursors and products that affect the metabolic equilibrium. We investigated the ...
    • Can the world afford to ignore biotechnology solutions that address food insecurity? 

      Berman Quintana, Judit; Zhu, Changfu; Pérez Massot, Eduard; Arjó Pont, Gemma; Zorrilla López, Uxue; Masip Vilà, Gemma; Banakar, Raviraj; Sanahuja Solsona, Georgina; Farré Martinez, Gemma; Miralpeix i Anglada, Bruna; Bai, Chao; Vamvaka, Evangelia; Sabalza Gallués, Maite; Twyman, Richard M.; Bassie Rene, Ludovic; Capell Capell, Teresa; Christou, Paul (Springer Verlag, 2013)
      Genetically engineered (GE) crops can be used as part of a combined strategy to address food insecurity, which is defined as a lack of sustainable access to safe and nutritious food. In this article, we discuss the ...
    • Carotenoid-enriched transgenic corn delivers bioavailable carotenoids to poultry and protects them against coccidiosis 

      Nogareda, Carmina; Moreno, José A.; Angulo Asensio, Eduardo; Sandmann, Gerhard; Portero Otín, Manuel; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul (John Wiley & SonsWiley-Blackwell, 2015)
      Carotenoids are health-promoting organic molecules that act as antioxidants and essential nutrients. We show that chickens raised on a diet enriched with an engineered corn variety containing very high levels of four key ...
    • Characteristics of genome editing mutations in cereal crops 

      Zhu, Changfu; Bortesi, Luisa; Baysal, Can; Twyman, Richard M.; Fischer, Rainer; Capell Capell, Teresa; Schillberg, Stefan; Christou, Paul (Elsevier, 2017)
      Designer nucleases allow the creation of new plant genotypes by introducing precisely-targeted double-strand breaks that are resolved by endogenous repair pathways. The major nuclease technologies are meganucleases, ...
    • Cloning and functional analysis of the promoters that upregulate carotenogenic gene expression during flower development in Gentiana lutea 

      Zhu, Changfu; Yang, Qingjie; Ni, Xiuzhen; Bai, Chao; Sheng, Yanmin; Shi, Lianxuan; Capell Capell, Teresa; Sandmann, Gerhard; Christou, Paul (Wiley, 2014)
      Over the last two decades, many carotenogenic genes have been cloned and used to generate metabolically engineered plants producing higher levels of carotenoids. However, comparatively little is known about the regulation ...
    • Cloning and Functional Characterization of the Maize (Zea mays L.) Carotenoid Epsilon Hydroxylase Gene 

      Chang, Shu; Berman Quintana, Judit; Sheng, Yanmin; Wang, Yingdian; Capell Capell, Teresa; Shi, Lianxuan; Ni, Xiuzhen; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Public Library of Science, 2015)
      The assignment of functions to genes in the carotenoid biosynthesis pathway is necessary to understand how the pathway is regulated and to obtain the basic information required for metabolic engineering. Few carotenoid ...
    • Cloning and functional characterization of the maize carotenoid isomerase and β-carotene hydroxylase genes and their regulation during endosperm maturation 

      Li, Qunrui; Farré Martinez, Gemma; Naqvi, Shaista; Breitenbach, Jürgen; Sanahuja Solsona, Georgina; Bai, Chao; Sandmann, Gerhard; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu (Springer Netherlands, 2010)
      In order to gain further insight into the partly-characterized carotenoid biosynthetic pathway in corn (Zea mays L.), we cloned cDNAs encoding the enzymes carotenoid isomerase (CRTISO) and β-carotene hydroxylase (BCH) using ...
    • Combined transcript, proteome, and metabolite analysis of transgenic maize seeds engineered for enhanced carotenoid synthesis reveals pleotropic effects in core metabolism 

      Decourcelle, Mathilde; Perez-Fons, Laura; Baulande, Sylvain; Steiger, Sabine; Couvelard, Linhdavanh; Hem, Sonia; Zhu, Changfu; Capell Capell, Teresa; Christou, Paul; Fraser, Paul; Sandmann, Gerhard (Oxford University Press, 2015-03-20)
      The aim of this study was to assess whether endosperm-specific carotenoid biosynthesis influenced core metabolic processes in maize embryo and endosperm and how global seed metabolism adapted to this expanded biosynthetic ...
    • Critical evaluation of strategies for mineral fortification of staple food crops 

      Gómez Galera, Sonia; Rojas, Eduard; Sudhakar, Duraialagaraja; Zhu, Changfu; Pelacho Aja, Ana Mª; Capell Capell, Teresa; Christou, Paul (Springer Verlag, 2010)
      Staple food crops, in particular cereal grains, are poor sources of key mineral nutrients. As a result, the world’s poorest people, generally those subsisting on a monotonous cereal diet, are also those most vulnerable ...
    • Differential accumulation of pelargonidin glycosides in petals at three different developmental stages of the orange-flowered gentian (Gentiana lutea L. var. aurantiaca) 

      Diretto, Gianfranco; Jin, Xin; Capell Capell, Teresa; Zhu, Changfu; Gómez Gómez, Lourdes (Public Library of Science, 2019)
      Corolla color in Gentiana lutea L. exhibits a yellow/orange variation. We previously demonstrated that the orange petal color of G. lutea L. var. aurantiaca is predominantly caused by newly synthesized pelargonidin ...
    • Engineering complex metabolic pathways in plants 

      Farré Martinez, Gemma; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul; Blancquaert, Dieter; Van Der Straeten, Dominique (Annual Reviews, 2014)
      Metabolic engineering can be used to modulate endogenous metabolic pathways in plants or introduce new metabolic capabilities in order to increase the production of a desirable compound or reduce the accumulation of ...
    • Engineering metabolic pathways in plants by multigene transformation 

      Zorrilla López, Uxue; Masip Vilà, Gemma; Arjó Pont, Gemma; Bai, Chao; Banakar, Raviraj; Bassie Rene, Ludovic; Berman Quintana, Judit; Farré Martinez, Gemma; Miralpeix i Anglada, Bruna; Pérez Massot, Eduard; Sabalza Gallués, Maite; Sanahuja Solsona, Georgina; Vamvaka, Evangelia; Twyman, Richard M.; Christou, Paul; Zhu, Changfu; Capell Capell, Teresa (University of the Basque Country Press, 2013)
      Metabolic engineering in plants can be used to increase the abundance of specific valuable metabolites, but single-point interventions generally do not improve the yields of target metabolites unless that product is ...
    • Fast quantitative method for the analysis of carotenoids in transgenic maize 

      Rivera Vélez, Sol Maiam; Vilaró, Francisca; Zhu, Changfu; Bai, Chao; Farré Martinez, Gemma; Christou, Paul; Canela i Garayoa, Ramon (American Chemical Society, 2013)
      A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass ...