Now showing items 1-17 of 17

    • A golden era—pro-vitamin A enhancement in diverse crops 

      Bai, Chao; Twyman, Richard M.; Farré Martinez, Gemma; Sanahuja Solsona, Georgina; Christou, Paul; Capell Capell, Teresa; Zhu, Changfu (Springer Verlag, 2011)
      Numerous crops have been bred or engineered to increase carotenoid levels in an effort to develop novel strategies that address vitamin A deficiency in the developing world. The pioneering work in rice (not covered in ...
    • A novel carotenoid, 4-keto-a-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callus 

      Breitenbach, Jürgen; Bai, Chao; Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu; Sandmann, Gerhard (Elsevier, 2014)
      Rice endosperm is devoid of carotenoids because the initial biosynthetic steps are absent. The early carotenogenesis reactions were constituted through co-transformation of endosperm-derived rice callus with phytoene ...
    • An in vitro system for the rapid functional characterization of genes involved in carotenoid biosynthesis and accumulation 

      Bai, Chao; Rivera Vélez, Sol Maiam; Medina Piles, Vicente; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Capell Capell, Teresa; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2014)
      We have developed an assay based on rice embryogenic callus for rapid functional characterization of metabolic genes. We validated the assay using a selection of well-characterized genes with known functions in the carotenoid ...
    • Biofortification of plants with altered antioxidant content and composition: genetic engineering strategies 

      Changfu, Zhu; Sanahuja Solsona, Georgina; Yuan, Dawei; Farré Martinez, Gemma; Arjó Pont, Gemma; Berman Quintana, Judit; Zorrilla López, Uxue; Banakar, Raviraj; Bai, Chao; Pérez Massot, Eduard; Bassie Rene, Ludovic; Capell Capell, Teresa; Christou, Paul (Wiley Open Access, 2013)
      Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death ...
    • Bottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor–product balance 

      Bai, Chao; Capell Capell, Teresa; Berman Quintana, Judit; Medina Piles, Vicente; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Wiley, 2016)
      The profile of secondary metabolites in plants reflects the balance of biosynthesis, degradation and storage, including the availability of precursors and products that affect the metabolic equilibrium. We investigated the ...
    • Can the world afford to ignore biotechnology solutions that address food insecurity? 

      Berman Quintana, Judit; Zhu, Changfu; Pérez Massot, Eduard; Arjó Pont, Gemma; Zorrilla López, Uxue; Masip Vilà, Gemma; Banakar, Raviraj; Sanahuja Solsona, Georgina; Farré Martinez, Gemma; Miralpeix i Anglada, Bruna; Bai, Chao; Vamvaka, Evangelia; Sabalza Gallués, Maite; Twyman, Richard M.; Bassie Rene, Ludovic; Capell Capell, Teresa; Christou, Paul (Springer Verlag, 2013)
      Genetically engineered (GE) crops can be used as part of a combined strategy to address food insecurity, which is defined as a lack of sustainable access to safe and nutritious food. In this article, we discuss the ...
    • Cloning and functional analysis of the promoters that upregulate carotenogenic gene expression during flower development in Gentiana lutea 

      Zhu, Changfu; Yang, Qingjie; Ni, Xiuzhen; Bai, Chao; Sheng, Yanmin; Shi, Lianxuan; Capell Capell, Teresa; Sandmann, Gerhard; Christou, Paul (Wiley, 2014)
      Over the last two decades, many carotenogenic genes have been cloned and used to generate metabolically engineered plants producing higher levels of carotenoids. However, comparatively little is known about the regulation ...
    • Cloning and functional characterization of the maize carotenoid isomerase and β-carotene hydroxylase genes and their regulation during endosperm maturation 

      Li, Qunrui; Farré Martinez, Gemma; Naqvi, Shaista; Breitenbach, Jürgen; Sanahuja Solsona, Georgina; Bai, Chao; Sandmann, Gerhard; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu (Springer Netherlands, 2010)
      In order to gain further insight into the partly-characterized carotenoid biosynthetic pathway in corn (Zea mays L.), we cloned cDNAs encoding the enzymes carotenoid isomerase (CRTISO) and β-carotene hydroxylase (BCH) using ...
    • Engineering metabolic pathways in plants by multigene transformation 

      Zorrilla López, Uxue; Masip Vilà, Gemma; Arjó Pont, Gemma; Bai, Chao; Banakar, Raviraj; Bassie Rene, Ludovic; Berman Quintana, Judit; Farré Martinez, Gemma; Miralpeix i Anglada, Bruna; Pérez Massot, Eduard; Sabalza Gallués, Maite; Sanahuja Solsona, Georgina; Vamvaka, Evangelia; Twyman, Richard M.; Christou, Paul; Zhu, Changfu; Capell Capell, Teresa (University of the Basque Country Press, 2013)
      Metabolic engineering in plants can be used to increase the abundance of specific valuable metabolites, but single-point interventions generally do not improve the yields of target metabolites unless that product is ...
    • Fast quantitative method for the analysis of carotenoids in transgenic maize 

      Rivera Vélez, Sol Maiam; Vilaró, Francisca; Zhu, Changfu; Bai, Chao; Farré Martinez, Gemma; Christou, Paul; Canela i Garayoa, Ramon (American Chemical Society, 2013)
      A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass ...
    • Functional characterization of the Gentiana lutea zeaxanthin epoxidase (GlZEP) promoter in transgenic tomato plants 

      Yang, Qingjie; Yuan, Dawei; Shi, Lianxuan; Capell Capell, Teresa; Bai, Chao; Wen, Nuan; Lu, Xiaodan; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu (Springer Verlag, 2010)
      The accumulation of carotenoids in plants depends critically on the spatiotemporal expression profiles of the genes encoding enzymes in the carotenogenic pathway. We cloned and characterized the Gentiana lutea zeaxanthin ...
    • Nutritious crops producing multiple carotenoids – a metabolic balancing act 

      Farré Martinez, Gemma; Bai, Chao; Twyman, Richard M.; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu (Elsevier, 2011)
      Plants and microbes produce multiple carotenoid pigments with important nutritional roles in animals. By unraveling the basis of carotenoid biosynthesis it has become possible to modulate the key metabolic steps in plants ...
    • The contribution of transgenic plants to better health through improved nutrition: opportunities and constraints 

      Pérez Massot, Eduard; Banakar, Raviraj; Gómez Galera, Sonia; Zorrilla López, Uxue; Sanahuja Solsona, Georgina; Arjó Pont, Gemma; Miralpeix i Anglada, Bruna; Vamvaka, Evangelia; Farré Martinez, Gemma; Rivera Vélez, Sol Maiam; Dashevskaya, Svetlana; Berman Quintana, Judit; Sabalza Gallués, Maite; Yuan, Dawei; Bai, Chao; Bassie Rene, Ludovic; Twyman, Richard M.; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu (Springer Verlag, 2013)
      Malnutrition is a prevalent and entrenched global socioeconomic challenge that reflects the combined impact of poverty, poor access to food, inefficient food distribution infrastructure, and an over-reliance on ...
    • The potential impact of plant biotechnology on the Millennium Development Goals 

      Yuan, Dawei; Bassie Rene, Ludovic; Sabalza Gallués, Maite; Miralpeix i Anglada, Bruna; Dashevskaya, Svetlana; Farré Martinez, Gemma; Rivera Vélez, Sol Maiam; Banakar, Raviraj; Bai, Chao; Sanahuja Solsona, Georgina; Arjó Pont, Gemma; Avilla, Eva; Zorrilla López, Uxue; Ugidos-Damboriena, Nerea; López, Alberto; Almacellas, David; Zhu, Changfu; Capell Capell, Teresa; Hahne, Gunther; Twyman, Richard M.; Christou, Paul (Springer Verlag, 2011)
      The eight Millennium Development Goals (MDGs) are international development targets for the year 2015 that aim to achieve relative improvements in the standards of health, socioeconomic status and education in the ...
    • The regulation of carotenoid pigmentation in flowers 

      Zhu, Changfu; Bai, Chao; Sanahuja Solsona, Georgina; Yuan, Dawei; Farré Martinez, Gemma; Naqvi, Shaista; Shi, Lianxuan; Capell Capell, Teresa; Christou, Paul (Elsevier, 2010)
      Carotenoids fulfill many processes that are essential for normal growth and development in plants, but they are also responsible for the breathtaking variety of red-to-yellow colors we see in flowers and fruits. Although ...
    • Travel advice on the road to carotenoids in plants 

      Farré Martinez, Gemma; Sanahuja Solsona, Georgina; Naqvi, Shaista; Bai, Chao; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul (Elsevier, 2010)
      The carotenoids are a major class of organic pigments produced in plants and microbes. They fulfill many essential physiological and developmental processes in plants, and also have important roles in animal health and ...
    • ZmPBF and ZmGAMYB transcription factors independently transactivate the promoter of the maize (Zea mays L.) β-carotene hydroxylase 2 gene 

      Jin, Xin; Bai, Chao; Bassie Rene, Ludovic; Nogareda, Carmina; Romagosa Clariana, Ignacio; Twyman, Richard M.; Christou, Paul; Zhu, Changfu (Wiley, 2018-12-01)
      The maize (Zea mays) enzyme b-carotene hydroxylase 2 (ZmBCH2) controls key steps in the conversion of b-carotene to zeaxanthin in the endosperm. The ZmBCH2 gene has an endosperm-preferred and developmentally regulated ...